Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells
Open Access
- 9 May 2020
- Vol. 9 (5), 604
- https://doi.org/10.3390/plants9050604
Abstract
Hechtian strands are thread-like structures in plasmolyzed plant cells that connect the cell wall to the plasma membrane. Although these strands were first observed more than 100 years ago, their physiological roles are largely unknown. Here, we used intracellular laser microdissection to examine the effects of disrupting Hechtian strands on plasmolyzed tobacco BY-2 cells. When we focused femtosecond laser pulses on Hechtian strands, targeted disruptions were induced, but no visible changes in cell morphology were detected. However, the calcofluor white signals from β-glucans was detected in plasmolyzed cells with disrupted Hechtian strands, whereas no signals were detected in untreated plasmolyzed cells. These results suggest that Hechtian strands play roles in sensing cell wall integrity.Funding Information
- Japan Society for the Promotion of Science (JP25291062, JP18H02466)
- Ministry of Education, Culture, Sports, Science and Technology (JP25114520, JP15H01235, JP24114002, JP18H05484, JP18H05489, JP18H05493)
- Exploratory Research for Advanced Technology (JPMJER1602)
This publication has 33 references indexed in Scilit:
- Building bridges: formin1 of Arabidopsis forms a connection between the cell wall and the actin cytoskeletonThe Plant Journal, 2011
- Plasmolysis and cell wall deposition in wheat root hairs under osmotic stressProtoplasma, 2009
- Identification of cell‐wall stress as a hexose‐dependent and osmosensitive regulator of plant responsesThe Plant Journal, 2009
- Molecular Interactions of Arabinogalactan Proteins with Cortical Microtubules and F-Actin in Bright Yellow-2 Tobacco Cultured CellsPlant Physiology, 2006
- Measurement of the Anchorage Force between GPI-Anchored Alkaline Phosphatase and Supported Membranes by AFM Force SpectroscopyLangmuir, 2005
- Membrane?wall attachments in plasmolysed plant cellsProtoplasma, 2004
- Tomato LeAGP‐1 is a plasma membrane‐bound, glycosylphosphatidylinositol‐anchored arabinogalactan‐proteinPhysiologia Plantarum, 2004
- Differential cytoplasm-plasma membrane-cell wall adhesion patterns and their relationships to hyphal tip growth and organelle motilityProtoplasma, 1997
- Behaviour of plasma membrane, cortical ER and plasmodesmata during plasmolysis of onion epidermal cellsPlant, Cell & Environment, 1994
- Plasmolysis, Plasmodesmata, and the Electrical Coupling of Oat Coleoptile CellsJournal of Experimental Botany, 1978