Transcriptome Comparison of Defense Responses in the Rice Variety ‘Jao Hom Nin’ Regarding Two Blast Resistant Genes, Pish and Pik
Open Access
- 29 May 2020
- Vol. 9 (6), 694
- https://doi.org/10.3390/plants9060694
Abstract
Jao Hom Nin (JHN) is a Thai rice variety with broad-spectrum resistant against rice blast fungus. JHN contains two rice blast resistant genes, Pish and Pik, located on chromosome 1 and on chromosome 11, respectively. To understand the blast resistance in JHN, the study of the defense mechanism related to the Pish and Pik genes is crucial. This study aimed to dissect defense response genes between the Pish and Pik genes using the RNA-seq technique. Differentially expressed genes (DEGs) of Pish and Pik backcross inbred lines were identified between 0 and 24 h after inoculation with rice blast spore suspension. The results showed that 1248 and 858 DEGs were unique to the Pish and Pik lines, respectively. The wall-associated kinase gene was unique to the Pish line and the zinc-finger-containing protein gene was unique to the Pik line. Pathogenicity-related proteins PR-4 and PR-10 were commonly found in both Pish and Pik lines. Moreover, DEGs functionally categorized in brassinosteriod, jasmonic acid, and salicylic acid pathways were detected in both Pish and Pik lines. These unique and shared genes in the Pish and Pik rice blast defense responses will help to dissect the mechanisms of plant defense and facilitate rice blast breeding programs.Keywords
Funding Information
- National Research Council of Thailand (-)
This publication has 55 references indexed in Scilit:
- Comparative Transcriptome Profiling of the Early Response to Magnaporthe oryzae in Durable Resistant vs Susceptible Rice (Oryza sativa L.) GenotypesPLOS ONE, 2012
- Simultaneous RNA-Seq Analysis of a Mixed Transcriptome of Rice and Blast Fungus InteractionPLOS ONE, 2012
- Comparative Analysis of Zinc Finger Proteins Involved in Plant Disease ResistancePLOS ONE, 2012
- Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and CufflinksNature Protocols, 2012
- The cell wall-associated kinases, WAKs, as pectin receptorsFrontiers in Plant Science, 2012
- Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-taggingBMC Plant Biology, 2010
- Characterization of a pathogenesis-related protein 4 (PR-4) induced in Capsicum chinense L3 plants with dual RNase and DNase activitiesJournal of Experimental Botany, 2010
- Genomic Diversity and Introgression in O. sativa Reveal the Impact of Domestication and Breeding on the Rice GenomePLOS ONE, 2010
- RNA‐Seq: A Method for Comprehensive Transcriptome AnalysisCurrent Protocols in Molecular Biology, 2010
- Plant Response to Bacterial Pathogens. Overlap between Innate and Gene-for-Gene Defense ResponsePlant Physiology, 2006