Comparative Analysis of Zinc Finger Proteins Involved in Plant Disease Resistance
Open Access
- 15 August 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (8), e42578
- https://doi.org/10.1371/journal.pone.0042578
Abstract
A meta-analysis was performed to understand the role of zinc finger domains in proteins of resistance (R) genes cloned from different crops. We analyzed protein sequences of seventy R genes of various crops in which twenty six proteins were found to have zinc finger domains along with nucleotide binding sites - leucine rice repeats (NBS-LRR) domains. We identified thirty four zinc finger domains in the R proteins of nine crops and were grouped into 19 types of zinc fingers. The size of individual zinc finger domain within the R genes varied from 11 to 84 amino acids, whereas the size of proteins containing these domains varied from 263 to 1305 amino acids. The biophysical analysis revealed that molecular weight of Pi54 zinc finger was lowest whereas the highest one was found in rice Pib zinc finger named as Transposes Transcription Factor (TTF). The instability (R2 = 0.95) and the aliphatic (R2 = 0.94) indices profile of zinc finger domains follows the polynomial distribution pattern. The pairwise identity analysis showed that the Lin11, Isl-1 & Mec-3 (LIM) zinc finger domain of rice blast resistance protein pi21 have 12.3% similarity with the nuclear transcription factor, X-box binding-like 1 (NFX) type zinc finger domain of Pi54 protein. For the first time, we reported that Pi54 (Pi-kh-Tetep), a rice blast resistance (R) protein have a small zinc finger domain of NFX type located on the C-terminal in between NBS and LRR domains of the R-protein. Compositional analysis depicted by the helical wheel diagram revealed the presence of a hydrophobic region within this domain which might help in exposing the LRR region for a possible R-Avr interaction. This domain is unique among all other cloned plant disease resistance genes and might play an important role in broad-spectrum nature of rice blast resistance gene Pi54.Keywords
This publication has 106 references indexed in Scilit:
- SMART 7: recent updates to the protein domain annotation resourceNucleic Acids Research, 2011
- Phylogenetic Study of Plant Q-type C2H2 Zinc Finger Proteins and Expression Analysis of Poplar Genes in Response to Osmotic, Cold and Mechanical StressesDNA Research, 2011
- Functional complementation of rice blast resistance gene Pi-k h (Pi54) conferring resistance to diverse strains of Magnaporthe oryzaeJournal of Plant Biochemistry and Biotechnology, 2011
- Functional analysis of a novel Cys2/His2-type zinc finger protein involved in salt tolerance in riceJournal of Experimental Botany, 2010
- RAD18 promotes DNA double-strand break repair during G1 phase through chromatin retention of 53BP1Nucleic Acids Research, 2009
- MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequencesBriefings in Bioinformatics, 2008
- Networks of WRKY transcription factors in defense signalingCurrent Opinion in Plant Biology, 2007
- Requirement of the C 3 HC 4 zinc RING finger of the Arabidopsis PEX10 for photorespiration and leaf peroxisome contact with chloroplastsProceedings of the National Academy of Sciences of the United States of America, 2007
- The Eight Amino-Acid Differences Within Three Leucine-Rich Repeats Between Pi2 and Piz-t Resistance Proteins Determine the Resistance Specificity to Magnaporthe griseaMolecular Plant-Microbe Interactions®, 2006
- High-resolution mapping, cloning and molecular characterization of the Pi-k h gene of rice, which confers resistance to Magnaporthe griseaMolecular Genetics and Genomics, 2005