Dual base editor catalyzes both cytosine and adenine base conversions in human cells
Top Cited Papers
- 1 June 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 38 (7), 856-860
- https://doi.org/10.1038/s41587-020-0527-y
Abstract
Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax’s activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.Funding Information
- National Natural Science Foundation of China (31925011)
- Science and Technology Commission of Shanghai Municipality (18411953500)
This publication has 25 references indexed in Scilit:
- Natural regulatory mutations elevate the fetal globin gene via disruption of BCL11A or ZBTB7A bindingNature Genetics, 2018
- Programmable base editing of A•T to G•C in genomic DNA without DNA cleavageNature, 2017
- KLF1 drives the expression of fetal hemoglobin in British HPFHBlood, 2017
- Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purityScience Advances, 2017
- Programmable editing of a target base in genomic DNA without double-stranded DNA cleavageNature, 2016
- ClinVar: public archive of interpretations of clinically relevant variantsNucleic Acids Research, 2015
- Improved vectors and genome-wide libraries for CRISPR screeningNature Methods, 2014
- Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleasesBioinformatics, 2014
- Establishment of Immortalized Human Erythroid Progenitor Cell Lines Able to Produce Enucleated Red Blood CellsPLOS ONE, 2013
- dbSNP: the NCBI database of genetic variationNucleic Acids Research, 2001