Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity
Top Cited Papers
Open Access
- 4 August 2017
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 3 (8), eaao4774-4774
- https://doi.org/10.1126/sciadv.aao4774
Abstract
Probing base editing outcomes leads to new C:G to T:A base editors with greater efficiency and product purity, and fewer indels.Keywords
Funding Information
- Defense Sciences Office, DARPA (award341255, HR0011-17-2-0049)
- HHMI (award341256, Liu Investigatorship)
- NIBIB (award341257, R01 EB022376)
- NIGMS (award341258, R35 GM118062)
This publication has 27 references indexed in Scilit:
- Improving the DNA specificity and applicability of base editing through protein engineering and protein deliveryNature Communications, 2017
- Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusionsNature Biotechnology, 2017
- CRISPR-Based Technologies for the Manipulation of Eukaryotic GenomesCell, 2016
- Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systemsScience, 2016
- Programmable editing of a target base in genomic DNA without double-stranded DNA cleavageNature, 2016
- Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editingScientific Reports, 2016
- DNA double strand break repair via non-homologous end-joining2013
- Structural basis for CRISPR RNA-guided DNA recognition by CascadeNature Structural & Molecular Biology, 2011
- Endogenous DNA double-strand breaks: Production, fidelity of repair, and induction of cancerProceedings of the National Academy of Sciences of the United States of America, 2003
- Homology-directed repair is a major double-strand break repair pathway in mammalian cellsProceedings of the National Academy of Sciences of the United States of America, 1998