A Novel Mouse HSF3 Has the Potential to Activate Nonclassical Heat-Shock Genes during Heat Shock
Open Access
- 1 January 2010
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 21 (1), 106-116
- https://doi.org/10.1091/mbc.e09-07-0639
Abstract
The heat-shock response is characterized by the expression of a set of classical heat-shock genes, and is regulated by heat-shock transcription factor 1 (HSF1) in mammals. However, comprehensive analyses of gene expression have revealed very large numbers of inducible genes in cells exposed to heat shock. It is believed that HSF1 is required for the heat-inducible expression of these genes although HSF2 and HSF4 modulate some of the gene expression. Here, we identified a novel mouse HSF3 (mHSF3) translocated into the nucleus during heat shock. However, mHSF3 did not activate classical heat-shock genes such as Hsp70. Remarkably, overexpression of mHSF3 restored the expression of nonclassical heat-shock genes such as PDZK3 and PROM2 in HSF1-null mouse embryonic fibroblasts (MEFs). Although down-regulation of mHSF3 expression had no effect on gene expression or cell survival in wild-type MEF cells, it abolished the moderate expression of PDZK3 mRNA and reduced cell survival in HSF1-null MEF cells during heat shock. We propose that mHSF3 represents a unique HSF that has the potential to activate only nonclassical heat-shock genes to protect cells from detrimental stresses.Keywords
This publication has 80 references indexed in Scilit:
- Heterotrimerization of Heat-Shock Factors 1 and 2 Provides a Transcriptional Switch in Response to Distinct StimuliMolecular Biology of the Cell, 2009
- Analysis of HSF4 Binding Regions Reveals Its Necessity for Gene Regulation during Development and Heat Shock Response in Mouse LensesJournal of Biological Chemistry, 2008
- Heat shock factor 1 regulates lifespan as distinct from disease onset in prion diseaseProceedings of the National Academy of Sciences of the United States of America, 2008
- The amphioxus genome and the evolution of the chordate karyotypeNature, 2008
- Human Alu RNA Is a Modular Transacting Repressor of mRNA Transcription during Heat ShockMolecular Cell, 2008
- A novel HSF1-mediated death pathway that is suppressed by heat shock proteinsThe EMBO Journal, 2006
- Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolutionNature, 2004
- Initial sequencing and comparative analysis of the mouse genomeNature, 2002
- Initial sequencing and analysis of the human genomeNature, 2001
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994