Environmental Enteropathy in Undernourished Pakistani Children: Clinical and Histomorphometric Analyses
Open Access
- 6 June 2018
- journal article
- research article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 98 (6), 1577-1584
- https://doi.org/10.4269/ajtmh.17-0306
Abstract
Despite nutrition interventions, stunting thought to be secondary to underlying environmental enteropathy (EE) remains pervasive among infants residing in resource-poor countries and remains poorly characterized. From a birth cohort of 380 children, 65 malnourished infants received 12 weeks of nutritional supplementation with ready-to-use therapeutic food (RUTF). Eleven children with insufficient response to RUTF underwent upper endoscopy with duodenal biopsies, which were compared with U.S., age-matched specimens for healthy, celiac disease, non-celiac villous atrophy, non-celiac intraepithelial lymphocytosis, and graft-versus-host disease patients. Of the 11 children biopsied, EE was found in 10 (91%) with one subject with celiac disease. Morphometry demonstrated decreased villus-to-crypt (V:C) ratios in EE relative to healthy and non-celiac lymphocytosis patients. Environmental enteropathy villus volumes were significantly decreased relative to healthy controls. In EE, average CD3+ cells per 100 epithelial cells and per 1,000 µm2 of lamina propria and the number of lamina propria CD20+ B-cell aggregates were increased relative to all other groups. Our results indicate that V:C ratios are reduced in EE but are less severe than in celiac disease. Environmental enteropathy intraepithelial and lamina propria T lymphocytosis is of greater magnitude than that in celiac disease. The increases in lamina propria B and T lymphocytes suggest that non-cytolytic lymphocytic activation may be a more prominent feature of EE relative to celiac disease. These results provide new insights into shared yet distinct histological and immunological features of EE and celiac disease in children.This publication has 32 references indexed in Scilit:
- Environmental enteropathy: critical implications of a poorly understood conditionTrends in Molecular Medicine, 2012
- Enteropathies in the Developing World: Neglected Effects on Global HealthThe American Journal of Tropical Medicine and Hygiene, 2012
- Worldwide prevalence of anaemia, WHO Vitamin and Mineral Nutrition Information System, 1993–2005Public Health Nutrition, 2008
- Tropical malabsorptionPublished by Oxford University Press (OUP) ,2006
- Chronic T Cell-Mediated Enteropathy in Rural West African Children: Relationship with Nutritional Status and Small Bowel FunctionPediatric Research, 2003
- Poor intestinal permeability in mildly stunted Nepali children: associations with weaning practices and Giardia lamblia infectionBritish Journal of Nutrition, 2002
- Innate Immunity, Gut Integrity, and Vitamin A in Gambian and Indian InfantsThe Journal of Infectious Diseases, 2000
- Asymptomatic environmental enteropathy among slum-dwelling infants.Journal of the American College of Nutrition, 1994
- Chronic Diarrhea and Malnutrition—Histology of the Small Intestinal LesionJournal of Pediatric Gastroenterology and Nutrition, 1991
- The villus architecture of the small intestine in the tropics: A necropsy studyThe Journal of Pathology, 1969