Hydroxyapatite bioactivated bacterial cellulose promotes osteoblast growth and the formation of bone nodules
Open Access
- 22 November 2012
- journal article
- Published by Springer Science and Business Media LLC in AMB Express
- Vol. 2 (1), 61
- https://doi.org/10.1186/2191-0855-2-61
Abstract
The goal of this study was to investigate the feasibility of bacterial cellulose (BC) scaffold to support osteoblast growth and bone formation. BC was produced by culturing Acetobacter xylinum supplemented with hydroxyapatite (HA) to form BC membranes (without HA) and BC/HA membranes. Membranes were subjected to X-ray photoelectron spectroscopy (XPS) analysis to determine surface element composition. The membranes were further used to evaluate osteoblast growth, alkaline phosphatase activity and bone nodule formation. BC was free of calcium and phosphate. However, XPS analysis revealed the presence of both calcium (10%) and phosphate (10%) at the surface of the BC/HA membrane. Osteoblast culture showed that BC alone was non-toxic and could sustain osteoblast adhesion. Furthermore, osteoblast adhesion and growth were significantly (p ≤0.05) increased on BC/HA membranes as compared to BC alone. Both BC and BC/HA membranes improved osteoconductivity, as confirmed by the level of alkaline phosphatase (ALP) activity that increased from 2.5 mM with BC alone to 5.3 mM with BC/HA. BC/HA membranes also showed greater nodule formation and mineralization than the BC membrane alone. This was confirmed by Alizarin red staining (ARS) and energy dispersive X-ray spectroscopy (EDX). This work demonstrates that both BC and BC/HA may be useful in bone tissue engineering.Keywords
This publication has 42 references indexed in Scilit:
- Bioactive Rosette Nanotube–Hydroxyapatite Nanocomposites Improve Osteoblast FunctionsTissue Engineering, Part A, 2012
- Osteoinduction of Human Mesenchymal Stem Cells by Bioactive Composite Scaffolds without Supplemental Osteogenic Growth FactorsPLOS ONE, 2011
- Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone RegenerationInternational Journal of Biomaterials, 2011
- Accelerated osteoblast mineralization on a conductive substrate by multiple electrical stimulationJournal of Bone and Mineral Metabolism, 2011
- Behavior of human chondrocytes in engineered porous bacterial cellulose scaffoldsJournal of Biomedical Materials Research Part A, 2010
- Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineeringBiomaterials, 2007
- Osteochondral repair using the combination of fibroblast growth factor and amorphous calcium phosphate/poly(l-lactic acid) hybrid materialsBiomaterials, 2007
- Prion Protein Aggregation and Neurotoxicity in Cortical NeuronsAnnals of the New York Academy of Sciences, 2007
- Hydroxyapatite/bacterial cellulose composites synthesized via a biomimetic routeMaterials Letters, 2006
- GROWTH STIMULATION OF AGED CELLS IN CULTUREActa Pathologica Microbiologica Scandinavica Section A Pathology, 1979