Mussel-inspired adhesive protein-based electrospun nanofibers reinforced by Fe(iii)–DOPA complexation
- 8 October 2014
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of Materials Chemistry B
- Vol. 3 (1), 112-118
- https://doi.org/10.1039/c4tb01496k
Abstract
Marine mussels utilize multiple bidentate complexes formed by Fe(III) and DOPA in a mussel adhesive protein (fp-1) to reinforce tough and elastic byssal fibers as a specialized underwater adhesive aid. In this study, mussel-inspired electrospun nanofibers were fabricated using a recombinant mussel adhesive protein (rfp-1), Fe(III)–DOPA complexes, and polycaprolactone. The mechanical properties of the fabricated nanofibers were reinforced by the Fe(III)–DOPA complex found in fp-1, which is a key component of the naturally occurring high-performance mussel fiber coating. Experimental results show that the stoichiometry of Fe(III)–DOPA complexes in the nanofibers could be controlled by buffer pH conditions and the stiffness of the nanofiber mat increased linearly with the concentration of the Fe(III)–DOPA complexes, as monitored by resonance Raman spectroscopy. This suggests the potential of Fe(III)–DOPA complexation as an effective strategy for modulating the mechanical properties of nanofibrous biomedical materials by using pH variations.Keywords
This publication has 43 references indexed in Scilit:
- Controlling Self-Renewal and Differentiation of Stem Cells via Mechanical CuesJournal of Biomedicine and Biotechnology, 2012
- Adhesion mechanism in a DOPA-deficient foot protein from green musselsSoft Matter, 2012
- Mussel-Inspired Adhesives and CoatingsAnnual Review of Materials Research, 2011
- pH-induced metal-ligand cross-links inspired by mussel yield self-healing polymer networks with near-covalent elastic moduliProceedings of the National Academy of Sciences of the United States of America, 2011
- Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in waterProceedings of the National Academy of Sciences of the United States of America, 2010
- Iron-Clad Fibers: A Metal-Based Biological Strategy for Hard Flexible CoatingsScience, 2010
- Metals and the Integrity of a Biological Coating: The Cuticle of Mussel ByssusLangmuir, 2008
- Functional electrospun nanofibrous scaffolds for biomedical applicationsAdvanced Drug Delivery Reviews, 2007
- Understanding Marine Mussel AdhesionMarine Biotechnology, 2007
- Single-molecule mechanics of mussel adhesionProceedings of the National Academy of Sciences of the United States of America, 2006