Antioxidant activity ofVigna unguiculataL. walp and hard-to-cookPhaseolus vulgarisL. protein hydrolysates
Open Access
- 1 August 2013
- journal article
- research article
- Published by Taylor & Francis Ltd in CyTA - Journal of Food
- Vol. 11 (3), 208-215
- https://doi.org/10.1080/19476337.2012.722687
Abstract
Recent research, using model systems, has shown that enzymatic hydrolysis of food proteins can make them act as direct scavengers of diverse free radicals. This study aimed at the characterization of protein hydrolysates with antioxidant properties from Vigna unguiculata L. walp and hard-to-cook Phaseolus vulgaris L. protein concentrates. The maximum values obtained for all the assays were 15.1 mM/mg of protein for Trolox-equivalent-antioxidative capacity, 98.2% for percentage of 2,2-diphenyl-1-picrylhydrazyl (DPPH) discoloration, 70.1% and 71.4% for iron and copper chelating activities, respectively. The reducing power of hydrolysates was higher than butylated hydroxytoluene (BHT) (control). The antioxidant activity of the hydrolysates may be a result of enzymatic hydrolysis generating peptides that exhibit this type of activity. The hydrolysates showed an increase of amino acids that have been associated with the biological activity of peptides with antioxidant activity, such as hydrophobic residues: Ala, Phe and Ile; hydrophilic: Lys and neutral: Ser and Gly.Keywords
This publication has 29 references indexed in Scilit:
- Structures and properties of antioxidative peptides derived from royal jelly proteinFood Chemistry, 2009
- Antioxidant Activity of Proteins and PeptidesCritical Reviews in Food Science and Nutrition, 2008
- Wet‐fractionation of Phaseolus lunatus seeds: partial characterization of starch and proteinJournal of the Science of Food and Agriculture, 2004
- Characterization of the Proteins from Vigna unguiculata SeedsJournal of Agricultural and Food Chemistry, 2004
- Agrobacterium-mediated co-transformation of a pea β-1,3-glucanase and chitinase genes in potato (Solanum tuberosum L. c.v. Russet Burbank) using a single selectable markerPlant Science, 2002
- Functional Properties of Flours and Protein Isolates from Phaseolus lunatus and Canavalia ensiformis SeedsJournal of Agricultural and Food Chemistry, 2001
- Production of Extensive Chickpea (Cicer arietinum L.) Protein Hydrolysates with Reduced Antigenic ActivityJournal of Agricultural and Food Chemistry, 1999
- Antioxidant Activity of Designed Peptides Based on the Antioxidative Peptide Isolated from Digests of a Soybean ProteinJournal of Agricultural and Food Chemistry, 1996
- Amino acid analysis by high-performance liquid chromatography after derivatization with diethyl ethoxymethylenemalonateJournal of Chromatography A, 1992
- Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine)Analytical Biochemistry, 1971