Activated platelets induce Weibel-Palade–body secretion and leukocyte rolling in vivo: role of P-selectin
Open Access
- 1 October 2005
- journal article
- Published by American Society of Hematology in Blood
- Vol. 106 (7), 2334-2339
- https://doi.org/10.1182/blood-2005-04-1530
Abstract
The presence of activated platelets and platelet-leukocyte aggregates in the circulation accompanies major surgical procedures and occurs in several chronic diseases. Recent findings that activated platelets contribute to the inflammatory disease atherosclerosis made us address the question whether activated platelets stimulate normal healthy endothelium. Infusion of activated platelets into young mice led to the formation of transient platelet-leukocyte aggregates and resulted in a several-fold systemic increase in leukocyte rolling 2 to 4 hours after infusion. Rolling returned to baseline levels 7 hours after infusion. Infusion of activated P-selectin-/- platelets did not induce leukocyte rolling, indicating that platelet P-selectin was involved in the endothelial activation. The endothelial activation did not require platelet CD40L. Leukocyte rolling was mediated solely by the interaction of endothelial P-selectin and leukocyte P-selectin glycoprotein ligand 1 (PSGL-1). Endothelial P-selectin is stored with von Willebrand factor (VWF) in Weibel-Palade bodies. The release of Weibel-Palade bodies on infusion of activated platelets was indicated by both elevation of plasma VWF levels and by an increase in the in vivo staining of endothelial P-selectin. We conclude that the presence of activated platelets in circulation promotes acute inflammation by stimulating secretion of Weibel-Palade bodies and P-selectin–mediated leukocyte rolling.Keywords
This publication has 58 references indexed in Scilit:
- Platelet P-selectin is required for pulmonary eosinophil and lymphocyte recruitment in a murine model of allergic inflammationBlood, 2005
- Vascular endothelial growth factor regulation of Weibel-Palade–body exocytosisBlood, 2005
- Effects of inflammatory cytokines on the release and cleavage of the endothelial cell–derived ultralarge von Willebrand factor multimers under flowBlood, 2004
- Relationship of homocysteine to markers of platelet and endothelial activation in ‘high risk’ hypertensives: a substudy of the Anglo-Scandinavian Cardiac Outcomes TrialInternational Journal of Cardiology, 2004
- Improved metabolic control decreases platelet activation markers in patients with type‐2 diabetesEuropean Journal of Clinical Investigation, 2004
- Platelet P-selectin facilitates atherosclerotic lesion developmentBlood, 2003
- State-of-the-Art Review : Effect of Sarpogrelate Hydrochloride on Platelet-Derived Microparticles and Various Soluble Adhesion Molecules in Diabetes MellitusClinical and Applied Thrombosis/hemostasis, 2000
- Humoral immune responses in CD40 ligand-deficient mice.The Journal of Experimental Medicine, 1994
- Platelets in Diabetes: The Role in the Hemostatic Regulation in AtherosclerosisSeminars in Thrombosis and Hemostasis, 1993
- Novel transcellular interaction: conversion of granulocyte‐derived leukotriene A4 to cysteinyl‐containing leukotrienes by human plateletsEuropean Journal of Biochemistry, 1988