Modulation of Canonical Transient Receptor Potential Channel 1 in the Proliferation of Oligodendrocyte Precursor Cells by the Golli Products of the Myelin Basic Protein Gene
Open Access
- 9 March 2011
- journal article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 31 (10), 3625-3637
- https://doi.org/10.1523/jneurosci.4424-10.2011
Abstract
Golli proteins, products of the myelin basic protein gene, function as a new type of modulator of intracellular Ca2+ levels in oligodendrocyte progenitor cells (OPCs). Because of this, they affect a number of Ca2+-dependent functions, such as OPC migration and process extension. To examine further the Ca2+ channels regulated by golli, we studied the store-operated Ca2+ channels (SOCCs) in OPCs and acute brain slice preparations from golli knock-out and golli-overexpressing mice. Our results showed that pharmacologically induced Ca2+ release from intracellular stores evoked a significant extracellular Ca2+ entry after store depletion in OPCs. They also indicated that, under these pharmacological conditions, golli promoted activation of Ca2+ influx by SOCCs in cultured OPCs as well as in tissue slices. The canonical transient receptor potential family of Ca2+ channels (TRPCs) has been postulated to be SOCC subunits in oligodendrocytes. Using a small interfering RNA knockdown approach, we provided direct evidence that TRPC1 is involved in store-operated Ca2+ influx in OPCs and that it is modulated by golli. Furthermore, our data indicated that golli is probably associated with TRPC1 at OPC processes. Additionally, we found that TRPC1 expression is essential for the effects of golli on OPC proliferation. In summary, our data indicate a key role for golli proteins in the regulation of TRPC-mediated Ca2+ influx, a finding that has profound consequences for the regulation of multiple biological processes in OPCs. More important, we have shown that extracellular Ca2+ uptake through TRPC1 is an essential component in the mechanism of OPC proliferation.This publication has 72 references indexed in Scilit:
- Evidence for an interaction between Golli and STIM1 in store-operated calcium entryBiochemical Journal, 2010
- Targeted Overexpression of a Golli-Myelin Basic Protein Isoform to Oligodendrocytes Results in Aberrant Oligodendrocyte Maturation and MyelinationASN Neuro, 2009
- Golli Myelin Basic Proteins Regulate Oligodendroglial Progenitor Cell Migration through Voltage-Gated Ca2+InfluxJournal of Neuroscience, 2009
- Essential Role for TRPC5 in Amygdala Function and Fear-Related BehaviorCell, 2009
- A point mutation in TRPC3 causes abnormal Purkinje cell development and cerebellar ataxia in moonwalker miceProceedings of the National Academy of Sciences of the United States of America, 2009
- Regulation of Store-Operated and Voltage-Operated Ca2+ Channels in the Proliferation and Death of Oligodendrocyte Precursor Cells by Golli ProteinsASN Neuro, 2009
- Orai proteins interact with TRPC channels and confer responsiveness to store depletionProceedings of the National Academy of Sciences of the United States of America, 2007
- Signaling proteins in raft-like microdomains are essential for Ca2+ wave propagation in glial cellsCell Calcium, 2007
- Store-Operated Calcium ChannelsPhysiological Reviews, 2005
- Cellular localization of TRPC3 channel in rat brain: preferential distribution to oligodendrocytesNeuroscience Letters, 2004