Gene cloning from yeast chromosome‐specific mini‐library Isolation of the SRP1 ‐related DNA sequence located on chromosome XV
- 18 July 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 234 (2), 421-425
- https://doi.org/10.1016/0014-5793(88)80129-7
Abstract
We have isolated and purified chromosome XV DNA molecules from the yeast S. cerevisiae using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. A chromosome-specific mini-library was constructed and the element of the SRP1 (serine-rich protein) related sequence family located within chromosome XV was isolated by in situ colony hybridization with an SRP1 probe. Results indicated that (i) a single-copy sequence homologous to SRP1 is present within chromosome XV; (ii) this sequence lies within the 2.3 kb HindIII fragment of the plasmid pXVAc6; (iii) the lack of a specific transcript from this SRP1 -related element suggests that it could be considered as a pseudogeneKeywords
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