A methylsulphonyl metabolite of a polychlorinated biphenyl can serve as a ligand for α2μ-globulin in rat and major-urinary-protein in mice
- 1 January 1990
- journal article
- research article
- Published by Informa UK Limited in Xenobiotica
- Vol. 20 (12), 1343-1352
- https://doi.org/10.3109/00498259009046632
Abstract
1. Kidneys from rats given an intraperitoneal dose of 4,4'-bis([3H]methylsulphonyl)-2,2',5,5'-tetrachlorobiphenyl [(CT3S02)2TCB)] contained (CT3S02)2TCB associated with a protein which has been isolated and characterized by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblot and immunodiffusion analysis to be α2μ-globulin (α2μ). 2. The same radioactive α2μ-globulin complex was isolated from urine of rats given an i.p. dose of (CT3S02)2TCB. This complex represented 12.3% (0.27% dose) and 9.3% (0.06% dose) of the radioactivity excreted in 0.24 h and 24-48 h urine, respectively. 3. The radioactivity was extractable from α2μ and characterized by t.l.c. (co-chromatography in two solvent systems) to be (CT3S02)2TCB. 4. A radioactive-protein complex was isolated from urine of mice given an i.p. dose of (CT3S02)2TCB. The radioactive-protein complex was characterized to be mouse major urinary protein (MUP) by SDS-PAGE, immunoblot and immunodiffusion analysis. This complex was not detected in mouse kidney. 5. Urinary excretion of radioactive-MUP complex represented 51.7% (2.3% dose) and 28% (1.3% dose) of the radioactivity excreted in 0.24 h and 24-48 h urine, respectively.Keywords
This publication has 23 references indexed in Scilit:
- Involvement of reversible binding to α2u-globulin in 1,4-dichlorobenzene-induced nephrotoxicityToxicology and Applied Pharmacology, 1989
- Occurrence and distribution of chlorinated aromatic methylsulfones and sulfoxides in biological samplesChemosphere, 1989
- Spatial differences and temporal trends of organochlorine compounds in biota from the northwestern hemisphereArchives of Environmental Contamination and Toxicology, 1988
- Phase transfer mediated synthesis of radiolabelled alkyl aryl ethers and sulphidesJournal of Labelled Compounds and Radiopharmaceuticals, 1987
- Evidence that methylsulfonyl metabolites of m-dichlorobenzene are causative substances of induction of hepatic microsomal drug-metabolizing enzymes by the parent compound in ratsToxicology and Applied Pharmacology, 1986
- Mercapturic acid pathway metabolites of xenobiotics: generation of potentially toxic metabolites during enterohepatic circulationTrends in Pharmacological Sciences, 1984
- Synthesis of 4,4′-BIS([3H]methylsulphonyl)-2,2′,5,5′-tetrachlorobiphenylJournal of Labelled Compounds and Radiopharmaceuticals, 1983
- Metabolism of 2,4′,5-Trichlorobiphenyl by the Mercapturic Acid PathwayScience, 1982
- Bronchial mucosal and kidney cortex affinity of 4- and 4,4′-substituted sulphur-containing derivatives of 2,2′-5,5′-tetrachlorobiphenyl in miceChemico-Biological Interactions, 1981
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976