Fatty Acids From the Cyanobacterium Microcystis Aeruginosa With Potent Inhibitory Effects on Fish Gill Na+/K+-ATPase Activity
Open Access
- 1 January 1998
- journal article
- Published by The Company of Biologists in Journal of Experimental Biology
- Vol. 201 (1), 81-89
- https://doi.org/10.1242/jeb.201.1.81
Abstract
Fatty acids from two strains of the cyanobacterium Microcystis aeruginosa, PCC 7820 (a strain that produces the hepatotoxin microcystin-LR, MC-LR) and CYA 43 (a strain that produces only small quantities of MC-LR), were extracted, partially characterised and tested for their inhibitory effect on the K+-dependent p-nitrophenol phosphatase (pNPPase) activity of tilapia (Oreochromis mossambicus) gill basolateral membrane. Thin-layer chromatography of the lipids from dichloromethane:methanol extracts of M. aeruginosa PCC 7820 and CYA 43, using diethylether:isopropanol:formic acid (100:4.5:2.5) as solvent, yielded five inhibitory products from M. aeruginosa 7820 and six from M. aeruginosa CYA 43. None of these products could be related to MC-LR. The inhibitory behaviour of the products mimics that of a slow, tight-binding inhibitor. The inhibitory activity is removed by incubation of extracts with fatty-acid-free bovine serum albumin (FAF-BSA). However, FAF-BSA only partially reversed the inhibition of K+-dependent pNPPase on fish gills pre-exposed to the extracted products. We conclude that M. aeruginosa strains PCC 7820 and CYA 43 produce fatty acids with potent inhibitory effects on K+-dependent pNPPase. The release of these products following lysis of cyanobacterial blooms may help to explain fish kills through a disturbance of gill functioning.Keywords
This publication has 30 references indexed in Scilit:
- Effect of irradiance on fatty acid, carotenoid, total protein composition and growth of Microcystis aeruginosaPhytochemistry, 1997
- Lipids of cyanobacteriumAphanizomenon flos-aquae and inhibition ofChlorella growthJournal of Chemical Ecology, 1994
- Inhibition of Zn2+-Dependent ATPase in Prostasome Membrane by IMonsaturated, Long-Chain Fatty AcidsUrologia Internationalis, 1992
- Effect of free fatty acids and detergents on H,K-ATPase. The steady-state ATP phosphorylation level and the orientation of the enzyme in membrane preparationsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1991
- Histopathological effects of microcystin-LR, a cyclic peptide toxin from the cyanobacterium (blue-green alga) Microcystis aeruginosa on common carp (Cyprinus carpio L.)Aquatic Toxicology, 1991
- Toxicity of a soluble peptide from Microcystis sp. to zooplankton and fishFreshwater Biology, 1990
- The effect of linoleic and arachidonic acid derivatives on calcium transport in vesicles from cardiac sarcoplasmic reticulumJournal of Molecular and Cellular Cardiology, 1988
- The toxicity of the cyanobacterium Microcystis aeruginosa to rainbow trout, Salmo gairdneri RichardsonJournal of Fish Diseases, 1985
- Inhibition of gastricBiochimica et Biophysica Acta (BBA) - Biomembranes, 1982
- Inhibition of (Na++K+)-stimulated ATPase of heart by fatty acidsJournal of Molecular and Cellular Cardiology, 1977