Platelet‐rich plasma enhances the initial mobilization of circulation‐derived cells for tendon healing
- 7 January 2008
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 215 (3), 837-845
- https://doi.org/10.1002/jcp.21368
Abstract
Circulation‐derived cells play a crucial role in the healing processes of tissue. In early phases of tendon healing processes, circulation‐derived cells temporarily exist in the wounded area to initiate the healing process and decrease in number with time. We assumed that a delay of time‐dependent decrease in circulation‐derived cells could improve the healing of tendons. In this study, we injected platelet‐rich plasma (PRP) containing various kinds of growth factors into the wounded area of the patellar tendon, and compared the effects on activation of circulation‐derived cells and enhancement of tendon healing with a control group (no PRP injection). To follow the circulation‐derived cells, we used a green fluorescent protein (GFP) chimeric rat expressing GFP in the circulating cells and bone marrow cells. In the PRP group, the numbers of GFP‐positive cells and heat‐shock protein (HSP47; collagen‐specific molecular chaperone)‐positive cells were significantly higher than in the control group at 3 and 7 days after injury. At the same time, the immunoreactivity for types I and III collagen was higher in the PRP group than in the control group at early phase of tendon healing. These findings suggest that locally injected PRP is useful as an activator of circulation‐derived cells for enhancement of the initial tendon healing process. J. Cell. Physiol. 215: 837–845, 2008.Keywords
This publication has 50 references indexed in Scilit:
- The Fate of Host and Graft Cells in Early Healing of Bone Tunnel after Tendon GraftThe American Journal of Sports Medicine, 2005
- Stromal Stem Cells and Platelet-Rich Plasma Improve Bone Allograft IntegrationPublished by Ovid Technologies (Wolters Kluwer Health) ,2005
- The role of bone marrow-derived cells in bone fracture repair in a green fluorescent protein chimeric mouse modelBiochemical and Biophysical Research Communications, 2005
- Behavior of Transplanted Bone Marrow-derived GFP Mesenchymal Cells in Osteochondral Defect as a Simulation of Autologous TransplantationJournal of Histochemistry & Cytochemistry, 2005
- Can bone marrow differentiate into renal cells?Pediatric Nephrology, 2002
- Collagen Fibril Diameters in the Rabbit Medial Collateral Ligament ScarConnective Tissue Research, 1997
- Proliferative and matrix synthesis response of canine anterior cruciate ligament fibroblasts submitted to combined growth factorsJournal of Orthopaedic Research, 1996
- Mesenchymal stem cellsJournal of Orthopaedic Research, 1991
- Experimental flexor tendon healing without adhesion formation — A new concept of tendon nutrition and intrinsic healing mechanisms: A preliminary reportThe Hand, 1976
- THE FIBROBLAST AND WOUND REPAIRBiological Reviews, 1968