Field Detection of Schistosoma japonicum Cercariae in Environmental Water Samples by Quantitative PCR
Open Access
- 15 March 2011
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 77 (6), 2192-2195
- https://doi.org/10.1128/aem.01561-10
Abstract
A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria.Keywords
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