Osteoblast Isolation from Murine Calvaria and Long Bones
- 14 October 2011
- book chapter
- Published by Springer Science and Business Media LLC
- Vol. 816, 19-29
- https://doi.org/10.1007/978-1-61779-415-5_2
Abstract
This chapter describes the isolation of primary mouse osteoblasts from adult mouse calvaria and long bones, as well as the process of isolation of bone cells from neonatal mouse calvaria. Osteoblasts from adult mouse bone are obtained as outgrowth from collagenase-treated bone pieces. Isolation of osteoblasts from neonatal calvaria is achieved by sequential enzymatic digestion of the bone matrix. Because of differences in origin and isolation method, each of the primary bone cell cultures described will have their own characteristics.Keywords
This publication has 14 references indexed in Scilit:
- Wnt/β-Catenin Signaling Is a Component of Osteoblastic Bone Cell Early Responses to Load-bearing and Requires Estrogen Receptor αOnline Journal of Public Health Informatics, 2007
- The production of nitric oxide and prostaglandin E2 by primary bone cells is shear stress dependentJournal of Biomechanics, 2001
- Different responsiveness of cells from adult and neonatal mouse bone to mechanical and biochemical challengeJournal of Cellular Physiology, 2001
- Pulsating Fluid Flow Increases Nitric Oxide (NO) Synthesis by Osteocytes but Not Periosteal Fibroblasts - Correlation with Prostaglandin UpregulationBiochemical and Biophysical Research Communications, 1995
- Osteoblast and osteoclast precursors in primary cultures of calvarial bone cellsThe Anatomical Record, 1986
- Bone formation and calcification by isolated osteoblastlike cells.The Journal of cell biology, 1982
- Biochemical and histological studies on various bone cell preparationsCalcified Tissue International, 1981
- Enzymatic isolation of osteoblasts from fetal rat calvariaThe Anatomical Record, 1977
- Separation of parathyroid hormone and calcitonin-sensitive cells from non-responsive bone cellsNature, 1974
- Bone Cells: Biochemical and Biological Studies after Enzymatic IsolationScience, 1964