A Primer for Morpholino Use in Zebrafish
- 1 March 2009
- journal article
- review article
- Published by Mary Ann Liebert Inc in Zebrafish
- Vol. 6 (1), 69-77
- https://doi.org/10.1089/zeb.2008.0555
Abstract
Morpholino oligonucleotides are the most common anti-sense “knockdown” technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.Keywords
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