Characterization of the De Novo Biosynthetic Enzyme of Platelet Activating Factor, DDT-Insensitive Cholinephosphotransferase, of Human Mesangial Cells
Open Access
- 1 January 2007
- journal article
- research article
- Published by Hindawi Limited in Mediators of Inflammation
- Vol. 2007, 1-10
- https://doi.org/10.1155/2007/27683
Abstract
Platelet activating factor (PAF), a potent inflammatory mediator, is implicated in several proinflammatory/inflammatory diseases such as glomerulonephritis, glomerulosclerosis, atherosclerosis, cancer, allergy, and diabetes. PAF can be produced by several renal cells under appropriate stimuli and it is thought to be implicated in renal diseases. The aim of this study is the characterization of DTT-insensitive cholinephosphotransferase (PAF-CPT) of human mesangial cell (HMC), the main regulatory enzyme of PAFde novobiosynthetic pathway. Microsomal fractions of mesangial cells were isolated and enzymatic activity and kinetic parameters were determined by TLC and in vitro biological test in rabbit washed platelets. The effect of bovine serum albumin (BSA), dithiothreitol (DTT), divalent cations (Mg2+and Ca2+), EDTA, and various chemicals on the activity of PAF-CPT of HMC was also studied. Moreover, preliminary in vitro tests have been performed with several anti-inflammatory factors such as drugs (simvastatin, IFNa, rupatadine, tinzaparin, and salicylic acid) and bioactive compounds of Mediterranean diet (resveratrol and lipids of olive oil, olive pomace, sea bass “Dicentrarchuslabrax,” and gilthead sea bream “Sparus aurata”). The results indicated that the above compounds can influence PAF-CPT activity of HMC.Keywords
Funding Information
- Greek State Scholarship’s Foundation
This publication has 48 references indexed in Scilit:
- Application of a TCA-precipitation method for the determination of 1-alkyl-sn-glycero-3-phosphate:Acetyl-CoA acetyltransferase in human renal tissueProstaglandins & Other Lipid Mediators, 2004
- Tinzaparin SodiumDrugs, 2004
- Pefabloc, 4-[2-aminoethyl]benzenesulfonyl fluoride, is a new, potent nontoxic and irreversible inhibitor of PAF-degrading acetylhydrolaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1996
- Modulation of Platelet-Activating Factor Synthesis by Recombinant Interferon-α in Human Renal Cell CarcinomaUrologia Internationalis, 1996
- Stable cell lines of T-SV40 immortalized human glomerular mesangial cellsKidney International, 1996
- PAFDrugs, 1991
- Acetylcholine and Dopamine Promote the Production of Platelet Activating Factor in Immature Cells of Chick Embryonic RetinaJournal of Neurochemistry, 1988
- Biosynthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor and a hypotensive lipid) by cholinephosphotransferase in various rat tissuesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Antihypertensive activity of an alkyl ether analog of phosphatidylcholineBiochemical and Biophysical Research Communications, 1979
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959