The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
Open Access
- 12 October 2010
- journal article
- research article
- Published by MyJove Corporation in Journal of Visualized Experiments
- No. 44,p. e2259
- https://doi.org/10.3791/2259
Abstract
Carboxyfluorescein succinimidyl ester (CFSE) is an effective and popular means to monitor lymphocyte division1-3. CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. Thus, when a CFSE-labeled cell divides, its progeny are endowed with half the number of carboxyfluorescein-tagged molecules and thus each cell division can be assessed by measuring the corresponding decrease in cell fluorescence via Flow cytometry. The capacity of CFSE to label lymphocyte populations with a high fluorescent intensity of exceptionally low variance, coupled with its low cell toxicity, make it an ideal dye to measure cell division. Since it is a fluorescein-based dye it is also compatible with a broad range of other fluorochromes making it applicable to multi-color flow cytometry. This article describes the procedures typically used for labeling mouse lymphocytes for the purpose of monitoring up to 8 cell divisions. These labeled cells can be used both for in vitro and in vivo studies.This publication has 4 references indexed in Scilit:
- Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and ProliferationCurrent Protocols in Immunology, 2009
- Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl esterNature Protocols, 2007
- Fluorescent dyes for lymphocyte migration and proliferation studiesImmunology & Cell Biology, 1999
- Determination of lymphocyte division by flow cytometryJournal of Immunological Methods, 1994