ENZYME-RELEASE AND SUPEROXIDE ANION PRODUCTION BY HUMAN ALVEOLAR MACROPHAGES STIMULATED WITH IMMUNOGLOBULIN-E
- 1 January 1980
- journal article
- research article
- Vol. 40 (2), 416-422
Abstract
Human alveolar macrophages specifically released lysosomal .beta.-glucuronidase and neutral proteases when successively incubated with Ig[immunoglobulin]E, and then, for 30 min, with anti-IgE. Superoxide anion [O2-] generation was obtained when anti-IgE-opsonized zymosan was added to IgE-incubated cells. Macrophages from smokers excreted twice as much enzymes and superoxide as cells from non-smokers. It was possible to induce the specific release of .beta.-glucuronidase with normal alveolar macrophages successively incubated with the serum of patients allergic to house dust or to grass pollen and then with the specific allergen. This characteristic opens the field to a direct test for allergic sera by analogy with the allergen-induced degranulation test of sensitized basophils.This publication has 24 references indexed in Scilit:
- Cytophilic binding of IgE to the macrophageCellular Immunology, 1979
- Cytophilic binding of IgE to the macrophageCellular Immunology, 1979
- Increased superoxide anion production by immunologically activated and chemically elicited macrophagesThe Journal of Experimental Medicine, 1978
- Interaction between IgE complexes and macrophages in the rat: a new mechanism of macrophage activationEuropean Journal of Immunology, 1977
- A subpopulation of normal human peripheral B lymphcytes that bind IgE.JCI Insight, 1977
- Immunoglobulins cytophilic for human lymphocytes, monocytes, and neutrophils.JCI Insight, 1975
- Specific IgE antibodies in immune adherence of normal macrophages to Schistosoma mansoni schistosomulesNature, 1975
- Antigen-Antibody Complexes in or on Eosinophils in Nasal SecretionsInternational Archives of Allergy and Immunology, 1975
- IGE AND IGGA ANTIBODY-MEDIATED RELEASE OF HISTAMINE FROM RAT PERITONEAL CELLSThe Journal of Experimental Medicine, 1971
- The microestimation of succinate and the extinction coefficient of cytochrome cBiochimica et Biophysica Acta, 1959