Karyotype analysis of Panax ginseng C.A.Meyer, 1843 (Araliaceae) based on rDNA loci and DAPI band distribution
Open Access
- 15 December 2012
- journal article
- Published by Pensoft Publishers in Comparative Cytogenetics
- Vol. 6 (4), 425-441
- https://doi.org/10.3897/compcytogen.v6i4.3740
Abstract
Ginseng has long been considered a valuable plant owing to its medicinal properties; however, genomic information based on chromosome characterization and physical mapping of cytogenetic markers has been very limited. Dual-color FISH karyotype and DAPI banding analyses of Panax ginseng C. A. Meyer, 1843 were conducted using 5S and 45S rDNA probes. The somatic chromosome complement was 2n=48 with lengths from 3.3 μm to 6.3 μm. The karyotype was composed of 12 metacentric, 9 submetacentric, and 3 subtelocentric pairs. The 5S rDNA probe localized to the intercalary region of the short arm of pair 11, while the 45S rDNA was located at the secondary constriction of the subtelocentric satellited chromosome 14. DAPI bands were clearly observed for most chromosomes, with various signal intensities and chromosomal distributions that consequently improved chromosome identification. As a result, all 24 chromosomes could be distinguished and numbers were assigned to each chromosome for the first time. The results presented here will be useful for the on-going ginseng genome sequencing and further molecular-cytogenetic studies and breeding programs of ginseng.Keywords
This publication has 58 references indexed in Scilit:
- EST-SSR Marker Sets for Practical Authentication of All Nine Registered Ginseng Cultivars in KoreaJournal of Ginseng Research, 2012
- Extensive chromosomal variation in a recently formed natural allopolyploid species,Tragopogon miscellus(Asteraceae)Proceedings of the National Academy of Sciences of the United States of America, 2012
- Dynamic distribution patterns of ribosomal DNA and chromosomal evolution in Paphiopedilum, a lady's slipper orchidBMC Plant Biology, 2011
- Pharmacology of ginsenosides: a literature reviewChinese Medicine, 2010
- Centromere repositioning in cucurbit species: Implication of the genomic impact from centromere activation and inactivationProceedings of the National Academy of Sciences of the United States of America, 2009
- Identification of a Maize Neocentromere in an Oat-Maize Addition LineCytogenetic and Genome Research, 2009
- The potential of high-resolution BAC-FISH in banana breedingEuphytica, 2008
- Evolution of rDNA in Nicotiana Allopolyploids: A Potential Link between rDNA Homogenization and EpigeneticsAnnals of Botany, 2008
- Detection and mapping of homologous and homoeologous segments in homoeologous groups of allotetraploid cotton by BAC-FISHBMC Genomics, 2007
- NOMENCLATURE FOR CENTROMERIC POSITION ON CHROMOSOMESHereditas, 1964