Characterization of porcine growth differentiation factor‐9 and its expression in oocyte maturation
- 9 October 2007
- journal article
- Published by Wiley in Molecular Reproduction and Development
- Vol. 75 (5), 707-714
- https://doi.org/10.1002/mrd.20810
Abstract
During follicular development, the importance of the interaction between oocytes and somatic cells is well‐known for maturation oocyte. Growth differentiation factor‐9 (gdf‐9) is thought to play an important role in such process. In this study, we characterized porcine gdf‐9 gene and investigate its expression during development. Also we determined its expression by different hormones during in vitro oocyte maturation. Overall length of pig gdf‐9 cDNA was cloned and its presumptive protein sequence of isolated cDNA has high homology to human or mouse GDF‐9 sequences. The gdf‐9 transcript was expressed in all stage of preimplantation embryos and fetuses, and was also expressed in the heart, brain, cumulus cells, spleen, ovary, and testis. Porcine GDF‐9 protein was specifically localized in in vitro matured oocyte and oocytes from adult ovary. In order to investigate the potential regulator of gdf‐9 gene expression, expression level of gdf‐9 gene was determined using serum‐free in vitro maturation (IVM) system. Supplementing with human chorionic gonadotrophin (hCG), pregnant mare serum gonadotrophin (PMSG), gonadotropin‐releasing hormone (GnRH), follicular stimulating hormone, luteinizing hormone (LH), or epidermal growth factor (EGF) did not affect level of gdf‐9 gene expression during ooycte maturation. In conclusion, porcine gdf‐9 gene is expressed in the porcine reproductive organ, especially in oocytes and embryos, and its expression is decreased during IVM. Mol. Reprod. Dev. 75: 707–714, 2008.Keywords
This publication has 27 references indexed in Scilit:
- Spatio-Temporal Expression of the Germ Cell Marker Genes MATER, ZAR1, GDF9, BMP15,andVASA in Adult Bovine Tissues, Oocytes, and Preimplantation Embryos1Biology of Reproduction, 2004
- Expression of Growth Differentiation Factor 9 Messenger RNA in Porcine Growing and Preovulatory Ovarian Follicles1Biology of Reproduction, 2004
- Age-associated alteration of gene expression patterns in mouse oocytesHuman Molecular Genetics, 2004
- Growth Differentiation Factor-9 and Stem Cell Factor Promote Primordial Follicle Formation in the Hamster: Modulation by Follicle-Stimulating Hormone1Biology of Reproduction, 2004
- Growth Differentiation Factor-9 Is Expressed by the Primate Follicle Throughout the Periovulatory Interval1Biology of Reproduction, 2003
- Intercellular Communication in the Mammalian Ovary: Oocytes Carry the ConversationScience, 2002
- Androgen receptor and follicle‐stimulating hormone receptor in the pig ovary during the follicular phase of the estrous cycle*Molecular Reproduction and Development, 2002
- Human Growth Differentiation Factor 9 (GDF-9) and Its Novel Homolog GDF-9B Are Expressed in Oocytes during Early FolliculogenesisJournal of Clinical Endocrinology & Metabolism, 1999
- Growth differentiation factor-9 is required during early ovarian folliculogenesisNature, 1996
- Synergetic effects of epidermal growth factor and gonadotropins on the cytoplasmic maturation of pig oocytes in a serum–free mediumZygote, 1995