Fusarium spp. associated with rice Bakanae: ecology, genetic diversity, pathogenicity and toxigenicity
- 25 February 2010
- journal article
- Published by Wiley in Environmental Microbiology
- Vol. 12 (3), 649-657
- https://doi.org/10.1111/j.1462-2920.2009.02105.x
Abstract
African and Asian populations of Fusarium spp. (Gibberella fujikuroi species complex) associated with Bakanae of rice (Oryzae sativa L.) were isolated from seeds and characterized with respect to ecology, phylogenetics, pathogenicity and mycotoxin production. Independent of the origin, Fusarium spp. were detected in the different rice seed samples with infection rate ranges that varied from 0.25% to 9%. Four Fusaria (F. andiyazi, F. fujikuroi, F. proliferatum and F. verticillioides) were found associated with Bakanae of rice. While three of the Fusaria were found in both African and Asian seed samples, F. fujikuroi was only detected in seed samples from Asia. Phylogenetic studies showed a broad genetic variation among the strains that were distributed into four different genetic clades. Pathogenicity tests showed that all strains reduced seed germination and possessed varying ability to cause symptoms of Bakanae on rice, some species (i.e. F. fujikuroi) being more pathogenic than others. The ability to produce fumonisins (FB(1) and FB(2)) and gibberellin A3 in vitro also differed according to the Fusarium species. While fumonisins were produced by most of the strains of F. verticillioides and F. proliferatum, gibberellin A3 was only produced by F. fujikuroi. Neither fumonisin nor gibberellin was synthesized by most of the strains of F. andiyazi. These findings provide new information on the variation within the G. fujikuroi species complex associated with rice seed and Bakanae disease.Keywords
This publication has 27 references indexed in Scilit:
- Fusarium Infections in Immunocompromised PatientsClinical Microbiology Reviews, 2007
- MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0Molecular Biology and Evolution, 2007
- A tale of two flatties: different responses of two terrestrial flatworms to past environmental climatic fluctuations at Tallaganda in montane southeastern AustraliaMolecular Ecology, 2006
- Fungal mycoflora and mycotoxins in Korean polished rice destined for humansInternational Journal of Food Microbiology, 2005
- Distribution of gibberellin biosynthetic genes and gibberellin production in the Gibberella fujikuroi species complexPhytochemistry, 2005
- Fungal metabolite screening: database of 474 mycotoxins and fungal metabolites for dereplication by standardised liquid chromatography–UV–mass spectrometry methodologyJournal of Chromatography A, 2003
- Fusarium andiyazi sp. nov., a New Species from SorghumMycologia, 2001
- Natural Occurrence of Fumonisins in Rice with Fusarium Sheath Rot DiseasePlant Disease, 1998
- Fusarium thapsinum (Gibberella thapsina): A New Species in Section Liseola from SorghumMycologia, 1997
- Variation in the Fusarium section Liseola: pathogenicity and genetic studies of isolates of Fusarium moniliforme Sheldon from different hosts in GhanaPlant Pathology, 1995