Golgi-derived CLASP-dependent microtubules control Golgi organization and polarized trafficking in motile cells
Open Access
- 23 August 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 11 (9), 1069-1080
- https://doi.org/10.1038/ncb1920
Abstract
Radial centrosomal microtubules have a role in Golgi positioning. Golgi-derived microtubules organized by CLASP are now shown to be required for the unique ribbon-like morphology of the Golgi by bringing together individual Golgi stacks. Disrupting Golgi-derived microtubules leads to defects in polarized secretion and directional cell-migration. Microtubules are indispensable for Golgi complex assembly and maintenance, which are integral parts of cytoplasm organization during interphase in mammalian cells. Here, we show that two discrete microtubule subsets drive two distinct, yet simultaneous, stages of Golgi assembly. In addition to the radial centrosomal microtubule array, which positions the Golgi in the centre of the cell, we have identified a role for microtubules that form at the Golgi membranes in a manner dependent on the microtubule regulators CLASPs. These Golgi-derived microtubules draw Golgi ministacks together in tangential fashion and are crucial for establishing continuity and proper morphology of the Golgi complex. We propose that specialized functions of these two microtubule arrays arise from their specific geometries. Further, we demonstrate that directional post-Golgi trafficking and cell migration depend on Golgi-associated CLASPs, suggesting that correct organization of the Golgi complex by microtubules is essential for cell polarization and motility.Keywords
This publication has 44 references indexed in Scilit:
- A Primary Role for Golgi Positioning in Directed Secretion, Cell Polarity, and Wound HealingMolecular Biology of the Cell, 2009
- Microtubule nucleation at the cis-side of the Golgi apparatus requires AKAP450 and GM130The EMBO Journal, 2009
- Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in ArabidopsisThe EMBO Journal, 2009
- Multiple Rab GTPase Binding Sites in GCC185 Suggest a Model for Vesicle Tethering at theTrans-GolgiMolecular Biology of the Cell, 2009
- Organelle positioning and cell polarityNature Reviews Molecular Cell Biology, 2008
- GRASP55 Regulates Golgi Ribbon FormationMolecular Biology of the Cell, 2008
- Molecular Mechanism of Mitotic Golgi Disassembly and Reassembly Revealed by a Defined Reconstitution AssayOnline Journal of Public Health Informatics, 2008
- Mechanisms of Mitotic Spindle Assembly and FunctionInternational Review of Cytology, 2008
- Asymmetric CLASP-Dependent Nucleation of Noncentrosomal Microtubules at the trans-Golgi NetworkDevelopmental Cell, 2007
- The Golgi complex: In vitro veritas?Cell, 1992