Extraction of Thiamylal in Serum Using Hydrophilic Acetonitrile with Subzero-Temperature and Salting-Out Methods
- 30 June 2004
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 76 (16), 4672-4675
- https://doi.org/10.1021/ac040065a
Abstract
For high-performance liquid chromatography (HPLC) of thiamylal, one of the barbiturates, the drug in serum samples was extracted by two alternative liquid−liquid extraction techniques using hydrophilic acetonitrile as a solvent and subzero-temperature and salting-out methods. Acetonitrile was mixed with the sample, separated by cooling at −20 °C or addition of sodium chloride, and injected directly into the HPLC apparatus. In both the methods, thiamylal was extracted effectively in the acetonitrile phase and pH adjustment of the sample was not required. The salting-out extraction method is rapid and would be suitable for quantitation of drugs in many samples. To avoid coextraction of added salt, the subzero-temperature extraction method was applied to identification of thiamylal by gas chromatography/mass spectrometry and liquid chromatography−tandem mass spectrometry.Keywords
This publication has 3 references indexed in Scilit:
- Subzero-Temperature Liquid−Liquid Extraction of Benzodiazepines for High-Performance Liquid ChromatographyAnalytical Chemistry, 1999
- Report of a Fatal Thiamylal Intoxication*Journal of Analytical Toxicology, 1991
- Toxicological analysis of thiamylal in biological materials by gas chromatography/mass spectrometryForensic Science International, 1988