Diets High in Heat-Treated Soybean Meal Reduce the Histamine-Induced Epithelial Response in the Colon of Weaned Piglets and Increase Epithelial Catabolism of Histamine
Open Access
- 19 November 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (11), e80612
- https://doi.org/10.1371/journal.pone.0080612
Abstract
We examined the influence of dietary fermentable protein (fCP) and fermentable carbohydrates (fCHO) on the colonic epithelial response to histamine in pigs. Thirty-two weaned piglets were fed 4 diets in a 2 × 2 factorial design with low fCP/low fCHO, low fCP/high fCHO, high fCP/low fCHO and high fCP/high fCHO. After 21-23 days, the pigs were killed and tissue from the proximal colon was stimulated with carbachol, histamine, PGE2 or sodium hydrogen sulphide in Ussing chambers. Changes in short-circuit current and tissue conductance were measured. Diamine oxidase, histamine N-methyltransferase, stem cell growth factor receptor, Fc-epsilon receptor I and cystic fibrosis transmembrane conductance regulator gene expression was determined. Activities of diamine oxidase and histamine N-methyltransferase and numbers of colonic mast cells were measured. The change in the short-circuit current in response to histamine was lower (P = 0.002) and tended to be lower for PGE2 (P = 0.053) in high fCP groups compared to low fCP groups, irrespective of fCHO. Additionally, the change in tissue conductance after the application of histamine was lower (P = 0.005) in the high fCP groups. The expression of histamine N-methyltransferase mRNA (P = 0.033) and the activities of diamine oxidase (P = 0.001) and histamine N-methyltransferase (P = 0.006) were higher with high fCP in comparison with low fCP. The expression of mast cell markers, stem cell growth factor receptor (P = 0.005) and Fc-epsilon receptor I (P = 0.049) was higher with high fCP diets compared to diets low in fCP, whereas the mast cell count did not differ between groups. The expression of the cystic fibrosis transmembrane conductance regulator was reduced (P = 0.001) with high fCP diets compared to low fCP diets. The lower epithelial response to histamine and PGE2 and elevated epithelial histamine inactivation suggests an adaptation to high fCP diets.Keywords
This publication has 44 references indexed in Scilit:
- Activation of rat intestinal mucosal mast cells by fat absorptionAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2012
- Allergic Mastocytic Gastroenteritis and Colitis: An Unexplained Etiology in Chronic Abdominal Pain and Gastrointestinal DysmotilityGastroenterology Research and Practice, 2012
- Histamine Derived from Probiotic Lactobacillus reuteri Suppresses TNF via Modulation of PKA and ERK SignalingPLOS ONE, 2012
- Strategic use of feed ingredients and feed additives to stimulate gut health and development in young pigsLivestock Science, 2010
- Actions of hydrogen sulphide on ion transport across rat distal colonBritish Journal of Pharmacology, 2009
- Enterocyte chloride and water secretion into the small intestine after enterotoxin challenge: Unifying hypothesis or intellectual dead end?Journal of Physiology and Biochemistry, 2008
- Acute inflammation alters bicarbonate transport in mouse ileumThe Journal of Physiology, 2007
- Determination of the activity of diamine oxidase in extremely small tissue samplesInflammation Research, 1995
- Biogene Amine in der Digesta von SchweinenArchives of Animal Nutrition, 1989
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976