Super-resolution Localization and Defocused Fluorescence Microscopy on Resonantly Coupled Single-Molecule, Single-Nanorod Hybrids
Open Access
- 29 January 2016
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Nano
- Vol. 10 (2), 2455-2466
- https://doi.org/10.1021/acsnano.5b07294
Abstract
Optical antennas made of metallic nanostructures dramatically enhance single-molecule fluorescence to boost the detection sensitivity. Moreover, emission properties detected at the optical far field are dictated by the antenna. Here we study the emission from molecule–antenna hybrids by means of super-resolution localization and defocused imaging. Whereas gold nanorods make single-crystal violet molecules in the tip’s vicinity visible in fluorescence, super-resolution localization on the enhanced molecular fluorescence reveals geometrical centers of the nanorod antenna instead. Furthermore, emission angular distributions of dyes linked to the nanorod surface resemble that of nanorods in defocused imaging. The experimental observations are consistent with numerical calculations using the finite-difference time-domain method.Keywords
Funding Information
- European Research Council (280064, 291593)
- Vlaamse Overheid (Meth/08/04)
- Federaal Wetenschapsbeleid (IAP-VI/27)
- Fonds Wetenschappelijk Onderzoek (1520915N, G.0B55.14)
- Japan Science and Technology Agency
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Herculesstichting (HER/08/21)
- Bijzonder Onderzoeksfonds (CREA2009, GOA2011/03, OT/12/059)
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