Ginsenoside Rg1 inhibits β-secretase activityin vitroand protects against Aβ-induced cytotoxicity in PC12 cells

Abstract

Ginsenoside Rg1 (1) is a major active component of Panax notoginseng, a Chinese herb widely used in traditional Chinese medicine to improve learning and memory function. Increasing evidence suggests that β-amyloid peptide (Aβ) plays a central role in the pathophysiology of Alzheimer's disease (AD). To elucidate the mechanism of 1 on improving the ability of learning and memory, we investigated whether 1 could affect Aβ generation or protect Aβ-induced neurotoxicity. The results showed that 1 could inhibit β-secretase activity in vitro and also protect the PC12 cells against injuries caused by exposure of PC12 cells to 50 μM Aβ_25-35 for 48 h. The cell death, LDH release, NO release, ROS production, lipid peroxidation, intracellular calcium elevation, and apoptosis are associated events induced by Aβ that can be rescued by 1 in PC12 cells. In conclusion, 1 may be a promising agent for AD, and the mechanism is related to β-secretase inhibition and protection against Aβ-induced cytotoxicity.