Advances in mass spectrometry based single-cell metabolomics
Open Access
- 23 October 2018
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 144 (3), 782-793
- https://doi.org/10.1039/c8an01581c
Abstract
Metabolomics has grown into a prominent field contributing to the molecular understanding of complex biological processes in both health and disease. Furthermore, single-cells are known to display metabolic differences between seemingly homogeneous populations of cells. Single-cell metabolomics attempts to analyze many cellular metabolites from single cells to understand phenotypic heterogeneity, which is a significant challenge due to the low analyte abundances and limited sample volumes. Label-free metabolite detection can be achieved with mass spectrometry, which is capable of simultaneously analyzing hundreds of metabolites. Herein, we review the recent advances in mass spectrometry based single-cell metabolomics, highlighting the current state-of-the-art within the last three years, and identify the challenges to move the field forward.Funding Information
- Stiftelsen för Strategisk Forskning (ICA-6)
- Vetenskapsrådet (621-2013-4231)
This publication has 60 references indexed in Scilit:
- Screening of Chlamydomonas reinhardtii Populations with Single-Cell Resolution by Using a High-Throughput Microscale Sample Preparation for Matrix-Assisted Laser Desorption Ionization Mass SpectrometryApplied and Environmental Microbiology, 2015
- Classification of Large Cellular Populations and Discovery of Rare Cells Using Single Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass SpectrometryAnalytical Chemistry, 2015
- Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryoProceedings of the National Academy of Sciences of the United States of America, 2015
- Defining the Metabolome: Size, Flux, and RegulationMolecular Cell, 2015
- Mass spectrometry based detection of glutathione with sensitivity for single-cell analysisRapid Communications in Mass Spectrometry, 2015
- Nanopipettes: probes for local sample analysisChemical Science, 2015
- Live Single-Cell Plant Hormone Analysis by Video-Mass SpectrometryPlant and Cell Physiology, 2015
- Latest applications of 3D ToF-SIMS bio-imagingBiointerphases, 2015
- Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometryRSC Advances, 2015
- Avoiding Misannotation of In-Source Fragmentation Products as Cellular Metabolites in Liquid Chromatography–Mass Spectrometry-Based MetabolomicsAnalytical Chemistry, 2015