Effect of Pressure on the Release of Radioactive Glycine and ?-Aminobutyric Acid from Spinal Cord Synaptosomes
- 1 November 1987
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 49 (5), 1571-1578
- https://doi.org/10.1111/j.1471-4159.1987.tb01029.x
Abstract
Exposure to high hydrostatic pressure produces neurological changes referred to as the high-pressure nervous syndrome (HPNS). Manifestations of HPNS include tremor, EEG changes, and convulsions. These symptoms suggest an alteration in synaptic transmission, particularly with inhibitory neural pathways. Because spinal cord transmission has been implicated in HPNS, this study investigated inhibitory neurotransmitter function in the cord at high pressure. Guinea pig spinal cord synaptosome preparations were used to study the effect of compression to 67.7 atmospheres absolute on [3H]glycine and [3H].gamma.-aminobutyric acid ([3H]GABA) release. Pressure was found to exert a significant suppressive effect on the depolarization-induced calcium-dependent release of glycine and GABA by these spinal cord presynaptic nerve terminals. This study suggests that decreased tonic inhibitory regulation at the level of the spinal cord contributes to the hyperexcitability observed in animals with compression to high pressure.Keywords
This publication has 35 references indexed in Scilit:
- Calcium-independent release of γ-aminobutyrate from nerve processes in the developing rabbit retinaNeuroscience, 1986
- Evidence in favor of a neurotransmitter role of glycine in the rat cerebral cortexBrain Research, 1982
- A method for studying synaptosomal release of neurotransmitter candidates, as evaluated by studies on cortical cholecystokinin octapeptide releasePeptides, 1982
- A rapid method for preparing synaptosomes: Comparison, with alternative proceduresBrain Research, 1981
- ON THE MECHANISM BY WHICH VERATRIDINE CAUSES A CALCIUM‐INDEPENDENT RELEASE OF γ‐AMINOBUTYRIC ACID FROM BRAIN SLICESBritish Journal of Pharmacology, 1981
- GABA and glycine transport in frog CNS: High affinity uptake and potassium-evoked release in vitroBrain Research, 1976
- Stimulus‐secretion coupling processes in brain: analysis of noradrenaline and gamma‐aminobutyric acid release.The Journal of Physiology, 1976
- Neurochemical Evidence for Glycine as a Transmitter and a Model for Its Intrasynaptosomal CompartmentationPublished by Springer Science and Business Media LLC ,1975
- Efflux of amino acid neurotransmitters from rat spinal cord slices. II. Factors influencing the electrically induced efflux of [14Cglycine and3H-GABABrain Research, 1971
- The hyperpolarization of spinal motoneurones by glycine and related amino acidsExperimental Brain Research, 1968