Radioreceptor Assay for 1α,25-Dihydroxyvitamin D 3
- 15 March 1974
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 183 (4129), 1089-1091
- https://doi.org/10.1126/science.183.4129.1089
Abstract
A competitive protein binding assay with a sensitivity of 80 picograms has been developed for 1α,25-dihydroxyvitamin D3, the hormonal form of vitamin D3. lα,25-Dihydroxyvitamin D3 displaced tritiated hormone from a cytosol-chromatin receptor preparation isolated from chick small intestine, providing a simple assay for the hormone. The concentration of lα, 25-dihydroxyvitamin D3 in human plasma, as determined by this assay, is approximately 6 nanograms per 100 milliliters; in patients with renal disease the concentration of this kidney-produced hormone is significantly lower.Keywords
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