Osteoblasts synthesize and respond to transforming growth factor-type beta (TGF-beta) in vitro.
Open Access
- 1 July 1987
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 105 (1), 457-463
- https://doi.org/10.1083/jcb.105.1.457
Abstract
Transforming growth factor-type beta (TGF-beta) has been identified as a constituent of bone matrix (Seyedin, S. M., A. Y. Thompson, H. Bentz, D. M. Rosen, J. M. McPherson, A. Conti, N. R. Siegel, G. R. Gallupi, and K. A. Piez, 1986, J. Biol. Chem. 261:5693-5695). We used both developing bone and bone-forming cells in vitro to demonstrate the cellular origin of this peptide. TGF-beta mRNA was detected by Northern analysis in both developing bone tissue and fetal bovine bone-forming cells using human cDNA probes. TGF-beta was shown to be synthesized and secreted by metabolically labeled bone cell cultures by immunoprecipitation from the medium. Further, TGF-beta activity was demonstrated in conditioned media from these cultures by competitive radioreceptor and growth promotion assays. Fetal bovine bone cells (FBBC) were found to have relatively few TGF-beta receptors (5,800/cell) with an extremely low Kd of 2.2 pM (high binding affinity). In contrast to its inhibitory effects on the growth of many cell types including osteosarcoma cell lines, TGF-beta stimulated the growth of subconfluent cultures of FBBC; it had little effect on the production of collagen by these cells. We conclude that bone-forming cells are a source for the TGF-beta that is found in bone, and that these cells may be modulated by this factor in an autocrine fashion.Keywords
This publication has 38 references indexed in Scilit:
- Transforming growth factors.1985
- Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization.Journal of Biological Chemistry, 1983
- Purification and initial characterization of a type beta transforming growth factor from human placenta.Proceedings of the National Academy of Sciences of the United States of America, 1983
- MORPHOLOGICAL AND BIOCHEMICAL-CHARACTERIZATION OF 4 CLONAL OSTEOGENIC-SARCOMA CELL-LINES OF RAT ORIGIN1983
- Mineral and collagen-binding proteins of fetal calf bone.Journal of Biological Chemistry, 1981
- New class of transforming growth factors potentiated by epidermal growth factor: isolation from non-neoplastic tissues.Proceedings of the National Academy of Sciences, 1981
- Activation of Adenosine 3',5'-Monophosphate-Dependent Protein Kinase in Normal and Malignant Bone Cells by Parathyroid Hormone, Prostaglandin E2, and Prostacyclin*Endocrinology, 1981
- Parathyroid Hormone-Responsive Clonal Cell Lines from Rat Osteosarcoma*Endocrinology, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970