Localization of Nopp140 within mammalian cells during interphase and mitosis
- 18 April 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Histochemistry and Cell Biology
- Vol. 132 (2), 129-140
- https://doi.org/10.1007/s00418-009-0599-8
Abstract
We investigated distribution of the nucleolar phosphoprotein Nopp140 within mammalian cells, using immunofluorescence confocal microscopy and immunoelectron microscopy. During interphase, three-dimensional image reconstructions of confocal sections revealed that nucleolar labelling appeared as several tiny spheres organized in necklaces. Moreover, after an immunogold labelling procedure, gold particles were detected not only over the dense fibrillar component but also over the fibrillar centres of nucleoli in untreated and actinomycin D-treated cells. Labelling was also consistently present in Cajal bodies. After pulse-chase experiments with BrUTP, colocalization was more prominent after a 10- to 15-min chase than after a 5-min chase. During mitosis, confocal analysis indicated that Nopp140 organization was lost. The protein dispersed between and around the chromosomes in prophase. From prometaphase to telophase, it was also detected in numerous cytoplasmic nucleolus-derived foci. During telophase, it reappeared in the reforming nucleoli of daughter nuclei. This strongly suggests that Nopp140 could be a component implicated in the early steps of pre-rRNA processing.Keywords
This publication has 67 references indexed in Scilit:
- Chromatin tethering effects of hNopp140 are involved in the spatial organization of nucleolus and the rRNA gene transcriptionJournal of Biomedical Science, 2008
- Characterization and Differential Nuclear Localization of Nopp140 and a Novel Nopp140-Like Protein in TrypanosomesEukaryotic Cell, 2006
- The Ribosomal RNA Processing Machinery Is Recruited to the Nucleolar Domain before RNA Polymerase I during Xenopus laevis DevelopmentThe Journal of cell biology, 2000
- In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive.The Journal of cell biology, 1996
- The rDNA transcription machinery is assembled during mitosis in active NORs and absent in inactive NORs.The Journal of cell biology, 1996
- The chromosome periphery during mitosisBioEssays, 1994
- Structure, function and assembly of the nucleolusTrends in Cell Biology, 1993
- Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin.The Journal of Experimental Medicine, 1991
- Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolusExperimental Cell Research, 1988
- Characterization of the nucleolar protein, B-36, using monoclonal antibodiesExperimental Cell Research, 1986