Survivin depletion inhibits tumor growth and enhances chemosensitivity in hepatocellular carcinoma
Open Access
- 22 July 2014
- journal article
- Published by Spandidos Publications in Molecular Medicine Reports
- Vol. 10 (4), 2025-2030
- https://doi.org/10.3892/mmr.2014.2413
Abstract
Survivin is a member of the inhibitor of apoptosis family, which has been suggested to be crucial in the control of cell division and inhibition of apoptosis. Expression of this protein has been observed in transformed cell lines and human tumor tissues, including those from colorectal cancer, but not in terminally differentiated adult tissues. Survivin mRNA expression has frequently been detected in hepatocellular carcinoma (HCC) and its protein expression has been demonstrated to be highly correlated with proliferation index rather than apoptotic index. The present study aimed to analyze the effect of survivin on the tumorigenicity and chemosensitivity of HCC via the establishment of an HCC cell line (PLC/PRF/5) with the stable knockdown of the survivin gene (PLC-k3). This cell line displayed significantly lower rates of survival and proliferation in assays of cell viability and proliferation, respectively, compared with those of the control cell line (PLC-v). In addition, PLC-k3 cells were more sensitive to cisplatin treatment, resulting in S phase arrest. These findings were further confirmed by an in vivo experiment. The data of the present study suggest that survivin is critical in promoting cell proliferation but not in inhibition of apoptosis, and enhances the chemosensitivity of HCC. Thus, the suppression of survivin expression in combination with cisplatin may contribute to the development of more effective treatments for HCC.published_or_final_versioKeywords
This publication has 18 references indexed in Scilit:
- Survivin expression is an independent poor prognostic marker in lung adenocarcinoma but not in squamous cell carcinomaVirchows Archiv, 2013
- Prognostic and Clinicopathological Significance of Survivin in Colorectal Cancer: A Meta-AnalysisPLOS ONE, 2013
- Quantitative analysis of apoptosis-related gene expression in hepatocellular carcinomaCancer, 2002
- The biology of apoptosisHuman Pathology, 2001
- The Survivin saga goes in vivoJCI Insight, 2001
- Proliferative and apoptotic activity in hepatocellular carcinoma and surrounding non-neoplastic liver tissuePflügers Archiv - European Journal of Physiology, 2001
- Survivin promotes cell proliferation in human hepatocellular carcinomaHepatology, 2000
- A Novel Anti-Apoptosis Gene: Re-expression of Survivin Messenger RNA as a Prognosis Marker in Non–Small-Cell Lung CancersJournal of Clinical Oncology, 1999
- The inhibitors of apoptosis (IAPs) and their emerging role in cancerOncogene, 1998
- A novel anti-apoptosis gene, survivin, expressed in cancer and lymphomaNature Medicine, 1997