Environmental microarray analyses of Antarctic soil microbial communities
- 20 November 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in The ISME Journal
- Vol. 3 (3), 340-351
- https://doi.org/10.1038/ismej.2008.111
Abstract
Antarctic ecosystems are fascinating in their limited trophic complexity, with decomposition and nutrient cycling functions being dominated by microbial activities. Not only are Antarctic habitats exposed to extreme environmental conditions, the Antarctic Peninsula is also experiencing unequalled effects of global warming. Owing to their uniqueness and the potential impact of global warming on these pristine systems, there is considerable interest in determining the structure and function of microbial communities in the Antarctic. We therefore utilized a recently designed 16S rRNA gene microarray, the PhyloChip, which targets 8741 bacterial and archaeal taxa, to interrogate microbial communities inhabiting densely vegetated and bare fell-field soils along a latitudinal gradient ranging from 51 °S (Falkland Islands) to 72 °S (Coal Nunatak). Results indicated a clear decrease in diversity with increasing latitude, with the two southernmost sites harboring the most distinct Bacterial and Archaeal communities. The microarray approach proved more sensitive in detecting the breadth of microbial diversity than polymerase chain reaction-based bacterial 16S rRNA gene libraries of modest size (~190 clones per library). Furthermore, the relative signal intensities summed for phyla and families on the PhyloChip were significantly correlated with the relative occurrence of these taxa in clone libraries. PhyloChip data were also compared with functional gene microarray data obtained earlier, highlighting numerous significant relationships and providing evidence for a strong link between community composition and functional gene distribution in Antarctic soils. Integration of these PhyloChip data with other complementary methods provides an unprecedented understanding of the microbial diversity and community structure of terrestrial Antarctic habitats.Keywords
This publication has 35 references indexed in Scilit:
- Climate change effects on soil arthropod communities from the Falkland Islands and the Maritime AntarcticSoil Biology and Biochemistry, 2008
- GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processesThe ISME Journal, 2007
- Urban aerosols harbor diverse and dynamic bacterial populationsProceedings of the National Academy of Sciences of the United States of America, 2007
- The effect of environmental change on vascular plant and cryptogam communities from the Falkland Islands and the Maritime AntarcticBMC Ecology, 2007
- Application of a High-Density Oligonucleotide Microarray Approach To Study Bacterial Population Dynamics during Uranium Reduction and ReoxidationApplied and Environmental Microbiology, 2006
- ORIGINAL ARTICLE: Diversity and biogeography of the Antarctic floraJournal of Biogeography, 2006
- Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARBApplied and Environmental Microbiology, 2006
- Unexpectedly High Bacterial Diversity in Arctic Tundra Relative to Boreal Forest Soils, Revealed by Serial Analysis of Ribosomal Sequence TagsApplied and Environmental Microbiology, 2005
- Ammonia-Oxidizing Bacteria: A Model for Molecular Microbial EcologyAnnual Review of Microbiology, 2001
- Structure and Function of Two Antarctic Terrestrial Moss CommunitiesEcological Monographs, 1981