Rapid Identification of Virulent Type I Strains of the Protozoan Pathogen Toxoplasma gondii by PCR-Restriction Fragment Length Polymorphism Analysis at the B1 Gene
- 1 January 2001
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (1), 398-400
- https://doi.org/10.1128/jcm.39.1.398-400.2001
Abstract
Sequence analysis at the 35-fold-repetitive B1 locus identified three restriction sites capable of discriminating type I (mouse-virulent) from type II or III (mouse-avirulent) strains of Toxoplasma gondii. B1 PCR-restriction fragment length polymorphism analysis of 8 type I, 17 type II, and 8 type III strains confirms the specificity of the assay. It should now be possible to ask whether strain genotype affects the severity and type of clinical disease in humans.Keywords
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