Prophylactic Intranasal Treatment with Fragments of 1,3-β-Glucanase Olive Pollen Allergen Prevents Airway Inflammation in a Murine Model of Type I Allergy
- 1 February 2006
- journal article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 139 (3), 175-180
- https://doi.org/10.1159/000091162
Abstract
Olive pollen is an important cause of allergy in Mediterranean countries. More than 50% of olive-pollen-allergic patients are sensitized against the 1,3-beta-glucanase Ole e 9. To date, prophylactic and therapeutic treatments using purified recombinant allergens have not been studied in animal models of olive pollen allergy. BALB/c mice were immunized against Ole e 9 combining intraperitoneal injections of the allergen in Al(OH)3 with airway allergen challenges. A prophylactic treatment was performed by intranasal administration of a mixture of the recombinant fragments of the allergen prior to Ole e 9 sensitization. Serum levels of specific IgE, IgG1, IgG2a and IgG2b were measured by ELISA, and total IgE levels by sandwich ELISA. Bronchoalveolar lavage and lungs from mice were collected to study airway inflammation by light microscopy. BALB/c mice immunized against Ole e 9 developed a predominantly Th2-like immune response with allergen-specific immunoglobulin induction and airway inflammation accompanied by the infiltration of eosinophils, lymphocytes, and neutrophils in the lung. Prophylactic treatment by intranasal application of the recombinant fragments of Ole e 9 avoids airway inflammation induced by sensitization with this allergen although the levels of Ole e 9-specific antibodies remain unchanged. Prophylactic intranasal treatment with recombinant fragments of Ole e 9 prevents airway inflammation triggered by immunization to this allergen in a murine model of type I allergy.Keywords
This publication has 23 references indexed in Scilit:
- 1,3‐β‐glucanases as candidates in latex–pollen–vegetable food cross‐reactivityClinical and Experimental Allergy, 2005
- A Major Allergen from Pollen Defines a Novel Family of Plant Proteins and Shows Intra- and Interspecie Cross-ReactivityPublished by The American Association of Immunologists ,2004
- The C-terminal segment of the 1,3-beta-glucanase Ole e 9 from olive (Olea europaea) pollen is an independent domain with allergenic activity: expression in Pichia pastoris and characterizationBiochemical Journal, 2003
- The future of antigen-specific immunotherapy of allergyNature Reviews Immunology, 2002
- Ole e 9, a Major Olive Pollen Allergen Is a 1,3-β-GlucanaseJournal of Biological Chemistry, 2001
- Development of Eosinophilic Airway Inflammation and Airway Hyperresponsiveness Requires Interleukin-5 but Not Immunoglobulin E or B LymphocytesAmerican Journal of Respiratory Cell and Molecular Biology, 1999
- High Levels of Olea europaea Pollen and Relation with Clinical FindingsInternational Archives of Allergy and Immunology, 1999
- Modulation of an Allergic Immune Response via the Mucosal Route in a Murine Model of Inhalative Type–I AllergyInternational Archives of Allergy and Immunology, 1999
- Oleaceae Pollinosis: A ReviewInternational Archives of Allergy and Immunology, 1996
- Systemic reactions occurring during immunotherapy with standardized pollen extractsJournal of Allergy and Clinical Immunology, 1992