Overexpression of the c-met Protooncogene in Human Gastric Carcinoma - Correlation to Clinical Features
Open Access
- 1 January 2001
- journal article
- research article
- Published by Medical Journals Sweden AB in Acta Oncologica
- Vol. 40 (5), 638-643
- https://doi.org/10.1080/028418601750444204
Abstract
Overexpression of hepatocyte growth factor receptor (c-met) has been detected in many human tumors. To investigate the possible involvement of c-met in human gastric carcinogenesis, we examined c-met expression in 45 patients with gastric carcinoma using Northern blot analysis, reverse transcription-polymerase chain reaction (RT-PCR), and immunohistochemical staining. The c-met mRNA expression was increased twofold and sevenfold in gastric carcinoma tissues compared to the adjacent normal tissues by Northern blot analysis and RT-PCR, respectively. In the immunohistochemical study, c-met protein was detected in 32 of 45 (71.1%) patients, with marked overexpression in gastric carcinoma compared with matched normal gastric tissues. The c-met-positive immunoreactivities were more frequently encountered in serosa-exposed and serosa-infiltrating gastric cancer (p = 0.003). In addition, tumor stage was another statistically significant parameter that was observed between the two groups (p = 0.02). Multivariate analyses revealed that the tumor stage (p = 0.014) and c-met overexpression (p = 0.031) were independently correlated with survival. These data suggest that overexpression of c-met may play a part in gastric carcinogenesis and may represent a prognostic factor for gastric cancer.Keywords
This publication has 13 references indexed in Scilit:
- Decreasing incidence of both major histologic subtypes of gastric adenocarcinoma – a population-based study in SwedenBritish Journal of Cancer, 2000
- The prognostic significance of amplification and overexpression of c-met and c-erb B-2 in human gastric carcinomasCancer, 1999
- Overexpression of c-met proto-oncogene associated with chromophilic renal cell carcinoma with papillary growth.Virchows Archiv, 1998
- Tyrosine kinase receptor-activated signal transduction pathways which lead to oncogenesisOncogene, 1998
- Transforming Growth Factor‐β and Hepatocyte Growth Factor Produced by Gastric Fibroblasts Stimulate the Invasiveness of Scirrhous Gastric Cancer CellsJapanese Journal of Cancer Research, 1997
- Expression of the c-met protooncogene in human hepatocellular carcinomaHepatology, 1994
- Frequent amplification of the c-met gene in scirrhous type stomach cancerBiochemical and Biophysical Research Communications, 1992
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987
- Mechanism of met oncogene activationCell, 1986
- Molecular cloning of a new transforming gene from a chemically transformed human cell lineNature, 1984