Relative Quantification in Imaging of a Peptide on a Mouse Brain Tissue by Matrix-Assisted Laser Desorption Ionization
- 24 April 2014
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 86 (10), 5131-5135
- https://doi.org/10.1021/ac500911x
Abstract
It is well-known that the number of analyte ions generated by matrix-assisted laser desorption ionization (MALDI) is not directly proportional to the analyte concentration at the irradiated spot. This is an obstacle to acquiring quantitatively meaningful maps for materials in a tissue by MALDI imaging. The problem worsens as the matrix suppression due to contaminants in the sample increases. In this work, we use a peptide as an example and show that we can overcome this problem by utilizing three guidelines derived from our recent studies on the generation of reproducible MALDI spectra. First is to acquire MALDI spectra under a temperature-controlled condition. Second is to keep the matrix suppression below an experimentally determined limit, and the third is to construct the image map using the peptide-to-matrix ion abundance ratio rather than the peptide ion abundance. The strategy works well for contaminated tissue samples and generates quantitatively meaningful maps. Also, it is demonstrated that a preposterous map can be generated when the peptide ion abundance is used in the construction of the map.Keywords
This publication has 27 references indexed in Scilit:
- Efficient Methods to Generate Reproducible Mass Spectra in Matrix-Assisted Laser Desorption Ionization of PeptidesJournal of the American Society for Mass Spectrometry, 2013
- Mass Spectrometric Imaging for Biomedical Tissue AnalysisChemical Reviews, 2010
- Optimization of Reflectron for Kinetic and Mechanistic Studies with Multiplexed Multiple Tandem (MSn) Time-of-flight Mass SpectrometryBulletin of the Korean Chemical Society, 2010
- Cluster secondary ion mass spectrometry of polymers and related materialsMass Spectrometry Reviews, 2009
- Temperature of Peptide Ions Generated by Matrix-Assisted Laser Desorption Ionization and Their Dissociation Kinetic ParametersThe Journal of Physical Chemistry B, 2009
- Solvent-Free Matrix Dry-Coating for MALDI Imaging of PhospholipidsJournal of the American Society for Mass Spectrometry, 2008
- Quantitative matrix-assisted laser desorption/ionization mass spectrometryBriefings in Functional Genomics and Proteomics, 2008
- MALDI imaging mass spectrometry: molecular snapshots of biochemical systemsNature Methods, 2007
- Thin Sectioning Improves the Peak Intensity and Signal-to-Noise Ratio in Direct Tissue Mass SpectrometryJournal of the Mass Spectrometry Society of Japan, 2006
- Peer Reviewed: Profiling and Imaging Proteins in Tissue Sections by MSAnalytical Chemistry, 2004