Acinetobacter baumannii is not Associated with Osteomyelitis in a Rat Model: A Pilot Study
- 1 January 2011
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Clinical Orthopaedics and Related Research
- Vol. 469 (1), 274-282
- https://doi.org/10.1007/s11999-010-1488-0
Abstract
Multidrug resistant Acinetobacter baumannii (MDR AB) with and without Staphylococcus aureus (SA) is a commonly isolated organism in infected segmental bone defects in combat-related trauma in Iraq and Afghanistan. Although MDR AB in visceral infections is a therapeutic challenge, control of infection appears more common for combat-related osteomyelitis. Using a rat model, we explored the virulence of MDR AB in segmental bone defects alone and in combination with SA. Segmental defects in 60 rat femurs were created, stabilized, and inoculated with MDR AB alone and 60 with MDR AB and SA. We performed qualitative and quantitative bacteriology and radiographic assessments at 2, 4, and 8 weeks for MDR AB and at 1, 2, and 3 weeks for MDR AB and SA. Quantitative bacteriology revealed a 3- to 5-log decrease in MDR AB from the initial inoculum. After polymicrobial inoculation, only 10 of 60 animals had positive cultures for MDR AB, whereas 59 of 60 animals had positive cultures for SA. Recovered SA were 2 to 5 log greater than the initial inoculum, while there again was a 3- to 5-log decrease in MDR AB. MDR AB alone did not cause bony lysis, but there was radiographic evidence of new bone formation in 67% of the segmental defects. Osteolysis was noted with MDR AB and SA. MDR AB did not appear to cause or contribute to clinically apparent osteomyelitis in this pilot study. Resolution of infections in combat-related segmental bone defects inoculated with MDR AB may be attributable to low virulence. Additional studies are needed to confirm low virulence and bone formation with MDR AB.Keywords
This publication has 21 references indexed in Scilit:
- Infectious Complications of Damage Control Orthopedics in War TraumaThe Journal of Trauma and Acute Care Surgery, 2009
- Efficacy of colistin‐impregnated beads to prevent multidrug‐resistant A. baumannii implant‐associated osteomyelitisJournal of Orthopaedic Research, 2009
- Multidrug-ResistantAcinetobacter baumanniiOsteomyelitis from IraqEmerging Infectious Diseases, 2008
- Osteomyelitis in Military Personnel Wounded in Iraq and AfghanistanThe Journal of Trauma and Acute Care Surgery, 2008
- Union of a Chronically Infected Internally Stabilized Segmental Defect in the Rat Femur After Debridement and Application of rhBMP-2 and Systemic AntibioticJournal of Orthopaedic Trauma, 2007
- An Outbreak of Multidrug-Resistant Acinetobacter baumannii-calcoaceticus Complex Infection in the US Military Health Care System Associated with Military Operations in IraqClinical Infectious Diseases, 2007
- Characterization of a chronic infection in an internally‐stabilized segmental defect in the rat femurJournal of Orthopaedic Research, 2005
- Considerations in Control and Treatment of Nosocomial Infections Due to Multidrug‐ResistantAcinetobacter baumanniiClinical Infectious Diseases, 2003
- Biofilms: Survival Mechanisms of Clinically Relevant MicroorganismsClinical Microbiology Reviews, 2002
- Osteogenic protein‐1 induced bone formation in an infected segmental defect in the rat femurJournal of Orthopaedic Research, 2002