Isolation and Characterization of Dually Reactive Strains of Group A Rotavirus from Hospitalized Children
- 1 November 2003
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (11), 5267-9
- https://doi.org/10.1128/jcm.41.11.5267-5269.2003
Abstract
Seven rotavirus strains dually reactive to serotype G1- and G2-specific monoclonal antibodies (MAbs) from hospitalized children with rotavirus diarrhea were culture adapted. Six strains were neutralized with G1 antiserum to a higher titer than that of G2, and one was neutralized with G1 and G2 antisera to equal titers. Of these, four strains were also neutralized with G6 antiserum. Five strains with short RNA pattern could not be serotyped, and the remaining two strains with long RNA pattern were serotyped as G1 strains. In addition, two strains showing dual reactivity to G2 and G4 MAbs and one G2-like strain from a nontypeable specimen were isolated. The dual reactivity of the isolates could not be attributed to mixed infections.Keywords
This publication has 20 references indexed in Scilit:
- Genomic Diversity of Group A Rotavirus Strains Infecting Humans in Eastern IndiaJournal of Clinical Microbiology, 2002
- Great Diversity of Group A Rotavirus Strains and High Prevalence of Mixed Rotavirus Infections in IndiaJournal of Clinical Microbiology, 2001
- Nigerian rotavirus serotype G8 could not be typedby PCR due to nucleotide mutation at the 3′ endof the primer binding siteArchiv für die gesamte Virusforschung, 1997
- A novel human rotavirus serotype with dual G5-G11 specificity.Journal of General Virology, 1997
- Review Of G And P Typing Results From A Global Collection Of Rotavirus Strains: Implications For Vaccine DevelopmentThe Journal of Infectious Diseases, 1996
- Epidemiology of symptomatic human rotaviruses in Bangalore and Mysore, India, from 1988 to 1994 as determined by electropherotype, subgroup and serotype analysisArchiv für die gesamte Virusforschung, 1996
- A porcine rotavirus strain with dual VP7 serotype specificityVirology, 1990
- Rotavirus serotypes causing acute diarrhoea in hospitalized children in Yogyakarta, Indonesia during 1978–1979Archiv für die gesamte Virusforschung, 1989
- Molecular epidemiology of human rotavirus infections based on genome segment variations in viral strainsJournal of Medical Virology, 1988
- Use of polyclonal and monoclonal antibodies and analysis of viral RNA in the detection of unusual group A human rotavirusesJournal of Medical Virology, 1988