EST analysis of gene expression in testis of the ascidian Ciona intestinalis
- 13 June 2002
- journal article
- research article
- Published by Wiley in Molecular Reproduction and Development
- Vol. 62 (4), 431-445
- https://doi.org/10.1002/mrd.10131
Abstract
To explore the gene expression underlying spermatogenesis, a large‐scale analysis has been done on the cDNAs from testis of the ascidian, Ciona intestinalis. A set of 5,461 expressed sequence tags was analyzed and grouped into 2,806 independent clusters. Approximately 30% of the clusters showed significant sequence matches to the proteins reported in DDBJ/GenBank/EMBL database including a set of proteins closely related to the gene regulation during spermatogenesis, functional and morphological changes of spermatogenic cells during spermiogenesis, and physiological functions of sperm, as well as those with housekeeping functions commonly expressed in other cells. Some clones show similarities to the proteins present in vertebrate lymphocytes, suggesting a primitive immune system in ascidians. We have also found some genes that are known to participate in hormonal regulation of spermatogenesis in vertebrates. The large majority of the genes expressed in Ciona testis show no significant matches to known proteins and the further analysis of these genes may shed new light on the molecular mechanism of spermatogenesis and sperm functions. Mol. Reprod. Dev. 62:431–445, 2002.Keywords
This publication has 71 references indexed in Scilit:
- Molecular chaperones in cilia and flagella: Implications for protein turnoverCell Motility, 1999
- Tctex2-Related Outer Arm Dynein Light Chain Is Phosphorylated at Activation of Sperm MotilityBiochemical and Biophysical Research Communications, 1999
- A protein associated with the manchette during rat spermiogenesis is encoded by a gene of the TBP-1-like subfamily with highly conserved ATPase and protease domainsMolecular Reproduction and Development, 1997
- A Novel Membrane-associated Metalloprotease, Ste24p, Is Required for the First Step of NH2-terminal Processing of the Yeast a-Factor PrecursorThe Journal of cell biology, 1997
- Cell Type-Specific Expression of hnRNP ProteinsExperimental Cell Research, 1995
- Attachment of the ascidian sperm surface egg receptor N‐acetylglucosaminidase to the cell membraneMolecular Reproduction and Development, 1994
- Purification and characterization of a vitelline coat lysin from Ciona intestinalis spermatozoaMolecular Reproduction and Development, 1992
- Sterol carrier protein 2 (non-specific lipid transfer protein) is localized in membranous fractions of Leydig cells and Sertoli cells but not in germ cellsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992
- Isolation, characterization, and localization of a sperm-bound N-acetylglucosaminidase that is indispensable for fertilization in the ascidian, Phallusia mammillataDevelopmental Biology, 1991
- Sperm Binding and Penetration during Ascidian Fertilization*Development, Growth & Differentiation, 1988