Agonist-induced endothelium-dependent relaxation in rat thoracic aorta may be mediated through cGMP.
- 1 March 1983
- journal article
- abstracts
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation Research
- Vol. 52 (3), 352-357
- https://doi.org/10.1161/01.res.52.3.352
Abstract
The present study investigates the hypothesis that endothelium-dependent relaxation of vascular smooth muscle may be mediated through the formation of cGMP. Relaxation of the rat thoracic aorta to acetylcholine, histamine, and Ca++ ionophore A23187 was associated with increased levels of cGMP in a time- and concentration-dependent manner, whereas cAMP levels were unaltered. Removal of the endothelium prevented relaxation to these agents and prevented the increased levels of cGMP. Removal of the endothelium after exposure to acetylcholine only partially decreased the elevated levels of cGMP, suggesting that the changes in cGMP occurred within the smooth muscle cells. Eicosatetraynoic acid, an inhibitor of lipoxygenase and cyclooxygenase, and quinacrine, an inhibitor of phospholipase, prevented and reversed acetylcholine-induced relaxation, respectively, and inhibited acetylcholine-induced increased levels of cGMP. In contrast, sodium nitroprusside-induced relaxation and increased levels of cGMP were independent of the presence of the endothelium, exposure to eicosatetraynoic acid, and quinacrine. The present results support the hypothesis that vascular smooth muscle relaxation induced by some agents is dependent on the presence of the endothelium and is mediated through the formation of an endothelial factor that increases cGMP levels in smooth muscle.Keywords
This publication has 17 references indexed in Scilit:
- Sodium nitroprusside-induced protein phosphorylation in intact rat aorta is mimicked by 8-bromo cyclic GMPProceedings of the National Academy of Sciences of the United States of America, 1982
- BRADYKININ‐INDUCED RELAXATION OF RENAL AND PULMONARY ARTERIES IS DEPENDENT UPON INTACT ENDOTHELIAL CELLSBritish Journal of Pharmacology, 1981
- Direct evidence against acetylcholine as the dilator transmitter in the cat cerebral arteryEuropean Journal of Pharmacology, 1980
- The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineNature, 1980
- Inhibitory effects of mepacrine and eicosatetraynoic acid on cyclic GMP elevations caused by calcium and hormonal factors in rat ductus deferensNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1980
- Anti-inflammatory and platelet anti-aggregant activity of phospholipase-A2 inhibitorsJournal of Pharmacy and Pharmacology, 1979
- Activation of guanylate cyclase by superoxide dismutase and hydroxyl radical: a physiological regulator of guanosine 3',5'-monophosphate formation.Proceedings of the National Academy of Sciences of the United States of America, 1977
- Sodium nitroprusside and other smooth muscle-relaxants increase cyclic GMP levels in rat ductus deferensNature, 1977
- Effects of decapitation, ether and pentobarbital on guanosine 3′,5′-phosphate and adenosine 3′,5′-phosphate levels in rat tissuesBiochimica et Biophysica Acta (BBA) - General Subjects, 1974