Biodegradation of 1,4-dioxane and transformation of related cyclic compounds by a newly isolated Mycobacterium sp. PH-06
- 17 December 2008
- journal article
- Published by Springer Science and Business Media LLC in Biodegradation
- Vol. 20 (4), 511-519
- https://doi.org/10.1007/s10532-008-9240-0
Abstract
A new bacterial strain PH-06 was isolated using enrichment culture technique from river sediment contaminated with 1,4-dioxane, and identified as belonging to the genus Mycobacterium based on 16S rRNA sequencing (Accession No. EU239889). The isolated strain effectively utilized 1,4-dioxane as a sole carbon and energy source and was able to degrade 900 mg/l 1,4-dioxane in minimal salts medium within 15 days. The key degradation products identified were 1,4-dioxane-2-ol and ethylene glycol, produced by monooxygenation. Degradation of 1,4-dioxane and concomitant formation of metabolites were demonstrated by GC/MS analysis using deuterium labeled 1,4-dioxane (1,4-dioxane-d8). In addition to 1,4-dioxane, this bacterium could also transform structural analogues such as 1,3-dioxane, cyclohexane and tetrahydrofuran when pre-grown with 1,4-dioxane as the sole growth substrate. Our results suggest that PH-06 can maintain sustained growth on 1,4-dioxane without any other carbon sources.Keywords
This publication has 26 references indexed in Scilit:
- Biodegradation of diphenyl ether and transformation of selected brominated congeners by Sphingomonas sp. PH-07Applied Microbiology and Biotechnology, 2007
- Pseudonocardia dioxanivorans sp. nov., a novel actinomycete that grows on 1,4-dioxaneInternational Journal of Systematic and Evolutionary Microbiology, 2005
- Classification of a polycyclic aromatic hydrocarbon-metabolizing bacterium, Mycobacterium sp. strain PYR-1, as Mycobacterium vanbaalenii sp. nov.International Journal of Systematic and Evolutionary Microbiology, 2002
- An Evaluation of the Common Mechanism Approach to the Food Quality Protection Act: Captan and Four Related Fungicides, a Practical ExampleInternational Journal of Toxicology, 2000
- Influence of protozoan grazing on contaminant biodegradationFEMS Microbiology Ecology, 1999
- Distribution of 1,4-dioxane in relation to possible sources in the water environmentScience of The Total Environment, 1999
- Photocatalytic degradation of 1,4-dioxane in aqueous solutionJournal of Photochemistry and Photobiology A: Chemistry, 1997
- Health Evaluation of 1,4-DioxaneToxicology and Industrial Health, 1996
- Oxidation and Biodegradability Enhancement of 1,4-Dioxane Using Hydrogen Peroxide and OzoneEnvironmental Science & Technology, 1994
- Degradation of dioxane, tetrahydrofuran and other cyclic ethers by an environmental Rhodococcus strainApplied Microbiology and Biotechnology, 1991