Cullin-based ubiquitin ligases: Cul3–BTB complexes join the family
Open Access
- 8 April 2004
- journal article
- review article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 23 (8), 1681-1687
- https://doi.org/10.1038/sj.emboj.7600186
Abstract
Cullin‐based E3 ligases target substrates for ubiquitin‐dependent degradation by the 26S proteasome. The SCF (Skp1–Cul1–F‐box) and ECS (ElonginC–Cul2–SOCS box) complexes are so far the best‐characterized cullin‐based ligases. Their atomic structure has been solved recently, and several substrates have been described in different organisms. In addition to Cul1 and Cul2, higher eucaryotic genomes encode for three other cullins: Cul3, Cul4, and Cul5. Recent results have shed light on the molecular composition and function of Cul3‐based E3 ligases. In these complexes, BTB‐domain‐containing proteins may bridge the cullin to the substrate in a single polypeptide, while Skp1/F‐box or ElonginC/SOCS heterodimers fulfill this function in the SCF and ECS complexes. BTB‐containing proteins are evolutionary conserved and involved in diverse biological processes, but their function has not previously been linked to ubiquitin‐dependent degradation. In this review, we present these new findings and compare the composition of Cul3‐based ligases to the well‐defined SCF and ECS ligases.Keywords
This publication has 49 references indexed in Scilit:
- Human De-Etiolated-1 Regulates c-Jun by Assembling a CUL4A Ubiquitin LigaseScience, 2004
- Targeting of protein ubiquitination by BTB–Cullin 3–Roc1 ubiquitin ligasesNature, 2003
- Release of Ubiquitin-Charged Cdc34-S∼Ub from the RING Domain Is Essential for Ubiquitination of the SCFCdc4-Bound Substrate Sic1Cell, 2003
- Structural Basis for Phosphodependent Substrate Selection and Orientation by the SCFCdc4 Ubiquitin LigaseCell, 2003
- Activation of UBC5 Ubiquitin-conjugating Enzyme by the RING Finger of ROC1 and Assembly of Active Ubiquitin Ligases by All CullinsPublished by Elsevier BV ,2002
- Structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase complexNature, 2002
- Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification PathwayScience, 2002
- Skp1p and the F-Box Protein Rcy1p Form a Non-SCF Complex Involved in Recycling of the SNARE Snc1p in YeastMolecular and Cellular Biology, 2001
- Leukemia translocation protein PLZF inhibits cell growth and expression of cyclin AOncogene, 1999
- The Elongin BC complex interacts with the conserved SOCS-box motif present in members of the SOCS, ras, WD-40 repeat, and ankyrin repeat familiesGenes & Development, 1998