Molecular identification and virulence of three Aeromonas hydrophila isolates cultured from infected channel catfish during a disease outbreak in west Alabama (USA) in 2009
- 9 May 2011
- journal article
- Published by Inter-Research Science Center in Diseases of Aquatic Organisms
- Vol. 94 (3), 249-253
- https://doi.org/10.3354/dao02332
Abstract
Three isolates (AL09-71, AL09-72, and AL09-73) of Aeromonas hydrophila were cultured from infected channel catfish Ictalurus punctatus during a disease outbreak in west Alabama, USA, in August 2009. Sequence analysis of the 16S-23S rDNA intergenic spacer region (ISR), cpn60, gyrB, and rpoD genes of the 3 strains revealed that the 3 strains were closely related to each other, sharing 97 to 99% nucleotide sequence similarities. However, ISR sequences of the 3 isolates from 2009 shared only 64% nucleotide sequences with AL98-C1B, a 1998 isolate of A. hydrophila cultured from diseased fish in Alabama. Sequences of cpn60, gyrB, and rpoD from the 3 isolates from 2009 shared 91 to 95% homologies with AL98-C1B. Based on both LD50 and LD95 values of intraperitoneal injection assays, the virulences of the 3 isolates from 2009 were not significantly different from each other, but were at least 200-fold more virulent than AL98-C1B, indicating that the 3 west Alabama isolates of A. hydrophila from 2009 were highly virulent to channel catfish.Keywords
This publication has 17 references indexed in Scilit:
- The reference strain Aeromonas hydrophicla CIP 57.50 should be reclassified as Aeromonas salmonicida CIP 57.50International Journal of Systematic and Evolutionary Microbiology, 2010
- Effect of chemotherapy, vaccines and immunostimulants on innate immunity of goldfish infected with Aeromonas hydrophilaDiseases of Aquatic Organisms, 2009
- Antagonism of Aeromonas hydrophila by propolis and its effect on the performance of Nile tilapia, Oreochromis niloticusFish & Shellfish Immunology, 2009
- Differences in the antibody response and survival of genetically different varieties of common carp (Cyprinus carpio L.) vaccinated with a commercial Aeromonas salmonicida/A. hydrophila vaccine and challenged with A. hydrophilaFish Physiology and Biochemistry, 2009
- Lack of agreement between biochemical and genetic identification of Aeromonas spp.APMIS, 2005
- Cloning, characterisation and expression of Aeromonas hydrophila major adhesinFish & Shellfish Immunology, 2004
- Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infectionAquaculture, 2003
- Oral administration of formalin-inactivated cells of Aeromonas hydrophila A3-51 controls infection by atypical A. salmonicida in goldfish, Carassius auratus (L.).Journal of Fish Diseases, 2003
- Is Aeromonas hydrophila the dominant motile Aeromonas species that causes disease outbreaks in aquaculture production in the Zhejiang Province of China?Diseases of Aquatic Organisms, 2001
- O-Serogrouping and surface components ofAeromonas hydrophilaandAeromonas jandaeipathogenic for eelsFEMS Microbiology Letters, 1994