Birthdays of retinal amacrine cell subtypes are systematically related to their molecular identity and soma position
- 13 August 2009
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 517 (5), 737-750
- https://doi.org/10.1002/cne.22200
Abstract
The mammalian retina contains six major cell types, several of which are divided into multiple molecularly and morphologically distinct subtypes. To understand how subtype diversity arises during development, we focused on amacrine interneurons in the mouse retina; ∼30 amacrine subtypes have been identified in mammals. We used antibody markers to identify the two main amacrine subsets—GABAergic and glycinergic—and further subdivided these groups into smaller subsets based on expression of neurotransmitter and transcription factor markers. We then used bromodeoxyuridine (BrdU) labeling to see whether amacrine subsets are born (become postmitotic) at different times, as is the case for lamina‐specified subsets of cortical projection neurons. We found that GABAergic amacrines are generated on average 2–3 days before glycinergic amacrines. Moreover, subsets of GABAergic amacrines are born at distinct times. We also found a strong correlation between amacrine cell birthday and soma position in the mature retina, another point of similarity with cortical projection neurons. This relationship raised the possibility that amacrine subtype identity is determined by signals that uncommitted cells receive after they migrate to their destinations. However, cells labeled with BrdU in vivo, then dissociated and allowed to develop in vitro, acquired the amacrine subtype‐specific markers appropriate for their birthdays, supporting the idea that they become specified near the time and place of their birth. Together, our results suggest that the birthdays of amacrine cells independently specify their destinations and subtype identities. J. Comp. Neurol. 517:737–750, 2009.Keywords
This publication has 75 references indexed in Scilit:
- Development and diversification of retinal amacrine interneurons at single cell resolutionProceedings of the National Academy of Sciences of the United States of America, 2009
- γ-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retinaDevelopment, 2008
- Development of Presynaptic Inhibition Onto Retinal Bipolar Cell Axon Terminals Is Subclass-SpecificJournal of Neurophysiology, 2008
- The determination of projection neuron identity in the developing cerebral cortexCurrent Opinion in Neurobiology, 2008
- Temporal order of bipolar cell genesis in the neural retinaNeural Development, 2008
- Expression of the LIM‐homeodomain protein Isl1 in the developing and mature mouse retinaJournal of Comparative Neurology, 2007
- Timing and topography of cell genesis in the rat retinaJournal of Comparative Neurology, 2004
- Vertebrate neural cell-fate determination: Lessons from the retinaNature Reviews Neuroscience, 2001
- Neuronal specification in the spinal cord: inductive signals and transcriptional codesNature Reviews Genetics, 2000
- Substance-P-like immunoreactive amacrine cells in the adult and the developing rat retinaDevelopmental Brain Research, 1992