The targeting information of the mitochondrial outer membrane isoform of cytochrome b5 is contained within the carboxyl‐terminal region
- 14 August 1995
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 370 (1-2), 69-74
- https://doi.org/10.1016/0014-5793(95)00797-d
Abstract
Two isoforms of mammalian cytochrome b5, which have homologous cytosolic amino-terminal catalytic domains, are located one on endoplasmic reticulum (ER b5) the other on mitochondrial outer membranes (OM b5). A cDNA coding for the previously unknown carboxyl-terminal domain of OM b5 was cloned and a chimera between the catalytic domain of ER b5 and the carboxyl-terminal region of OM b5 was expressed in cultured mammalian cells. The chimera localized to mitochondria, indicating that the carboxyl-terminal 43 amino acids of OM b5 contain sufficient information to target the catalytic domain of ER b5 to the mitochondrial outer membrane.Keywords
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